miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
30961 3' -54.4 NC_006556.1 + 13781 1.14 0.000169
Target:  5'- aCCAACCACCACCCGCCACUCGAAUCCu -3'
miRNA:   3'- -GGUUGGUGGUGGGCGGUGAGCUUAGG- -5'
30961 3' -54.4 NC_006556.1 + 1355 0.72 0.207438
Target:  5'- aCAGCCACggauCACCCGCCaaguccaacauGCUUGAGcCCu -3'
miRNA:   3'- gGUUGGUG----GUGGGCGG-----------UGAGCUUaGG- -5'
30961 3' -54.4 NC_006556.1 + 12446 0.68 0.393034
Target:  5'- gCAACCACCAuaUCgGCCAUga-AGUCCa -3'
miRNA:   3'- gGUUGGUGGU--GGgCGGUGagcUUAGG- -5'
30961 3' -54.4 NC_006556.1 + 7781 0.67 0.453606
Target:  5'- aCGugUcCCACCCGCCACagcCGAAa-- -3'
miRNA:   3'- gGUugGuGGUGGGCGGUGa--GCUUagg -5'
30961 3' -54.4 NC_006556.1 + 7486 0.66 0.495581
Target:  5'- cCCAACCGCgucaguaUACCgGCUAUguaaaGggUCCu -3'
miRNA:   3'- -GGUUGGUG-------GUGGgCGGUGag---CuuAGG- -5'
30961 3' -54.4 NC_006556.1 + 11782 0.66 0.496681
Target:  5'- cCCAACUACCGCCCcaacaacuaaaGCCAacgcCGAAa-- -3'
miRNA:   3'- -GGUUGGUGGUGGG-----------CGGUga--GCUUagg -5'
30961 3' -54.4 NC_006556.1 + 8982 0.66 0.530158
Target:  5'- cCCAGaaauccCCACCACCgGUaAUggGAAUCCa -3'
miRNA:   3'- -GGUU------GGUGGUGGgCGgUGagCUUAGG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.