Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30962 | 3' | -52.6 | NC_006556.1 | + | 10686 | 0.66 | 0.569928 |
Target: 5'- uGGCCAuuUGGCUGUcGGauaauaGCGCGgCGGc -3' miRNA: 3'- -UCGGUuuAUCGGCAaUC------UGCGCgGUC- -5' |
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30962 | 3' | -52.6 | NC_006556.1 | + | 10202 | 0.66 | 0.557972 |
Target: 5'- cGCCAGAacGCCG-UAGACcaccaaaGCCAGa -3' miRNA: 3'- uCGGUUUauCGGCaAUCUGcg-----CGGUC- -5' |
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30962 | 3' | -52.6 | NC_006556.1 | + | 10980 | 0.66 | 0.534286 |
Target: 5'- cAGCCA---AGCCGUcaagggugaccUAGAgGCGCUugAGg -3' miRNA: 3'- -UCGGUuuaUCGGCA-----------AUCUgCGCGG--UC- -5' |
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30962 | 3' | -52.6 | NC_006556.1 | + | 6496 | 0.67 | 0.488079 |
Target: 5'- gGGCUAugaGGCCGccaaggUGGugGCGCCc- -3' miRNA: 3'- -UCGGUuuaUCGGCa-----AUCugCGCGGuc -5' |
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30962 | 3' | -52.6 | NC_006556.1 | + | 10719 | 0.67 | 0.476818 |
Target: 5'- cAGCCAAAUGGCCaaaGUgccUAGAUacaaCGCCAa -3' miRNA: 3'- -UCGGUUUAUCGG---CA---AUCUGc---GCGGUc -5' |
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30962 | 3' | -52.6 | NC_006556.1 | + | 8043 | 0.68 | 0.454692 |
Target: 5'- uGGCCAGAUauGGCCGaaauGGCGaauugGCCGGu -3' miRNA: 3'- -UCGGUUUA--UCGGCaau-CUGCg----CGGUC- -5' |
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30962 | 3' | -52.6 | NC_006556.1 | + | 6629 | 0.68 | 0.433134 |
Target: 5'- aAGCCGAAauUGGCCGguccuuGGCaCGCCAc -3' miRNA: 3'- -UCGGUUU--AUCGGCaau---CUGcGCGGUc -5' |
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30962 | 3' | -52.6 | NC_006556.1 | + | 11156 | 0.69 | 0.37222 |
Target: 5'- cGCC-AAUAGCCGcuucUUGGACagcacgccuaGCGCCAa -3' miRNA: 3'- uCGGuUUAUCGGC----AAUCUG----------CGCGGUc -5' |
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30962 | 3' | -52.6 | NC_006556.1 | + | 13235 | 0.71 | 0.292361 |
Target: 5'- uAGCCAAcgGGCCGgUGGACauaGCCGu -3' miRNA: 3'- -UCGGUUuaUCGGCaAUCUGcg-CGGUc -5' |
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30962 | 3' | -52.6 | NC_006556.1 | + | 14947 | 0.73 | 0.220051 |
Target: 5'- cGCUAGGUaucuGGCCuaUAGAgGCGCCAGg -3' miRNA: 3'- uCGGUUUA----UCGGcaAUCUgCGCGGUC- -5' |
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30962 | 3' | -52.6 | NC_006556.1 | + | 16987 | 0.76 | 0.124244 |
Target: 5'- uGGCCAcAUAGCCG-UAGGC-CGCCAa -3' miRNA: 3'- -UCGGUuUAUCGGCaAUCUGcGCGGUc -5' |
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30962 | 3' | -52.6 | NC_006556.1 | + | 17782 | 0.77 | 0.109743 |
Target: 5'- cGGCCAAGUcuuuaGGCCGUauaugcUGGGCGCGCUg- -3' miRNA: 3'- -UCGGUUUA-----UCGGCA------AUCUGCGCGGuc -5' |
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30962 | 3' | -52.6 | NC_006556.1 | + | 17234 | 1.1 | 0.000409 |
Target: 5'- cAGCCAAAUAGCCGUUAGACGCGCCAGa -3' miRNA: 3'- -UCGGUUUAUCGGCAAUCUGCGCGGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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