Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 30965 | 3' | -52.8 | NC_006556.1 | + | 3780 | 0.66 | 0.562039 |
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Target: 5'- -gGCGAUACUAUgaCGGCCAAuacGGGagaGUc -3' miRNA: 3'- caUGCUGUGAUG--GCCGGUUu--UCCg--CA- -5' |
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| 30965 | 3' | -52.8 | NC_006556.1 | + | 4206 | 0.67 | 0.508283 |
|
Target: 5'- -gACGAUACUGaCGGCCGcgguagaggguccuAGGGCGg -3' miRNA: 3'- caUGCUGUGAUgGCCGGUu-------------UUCCGCa -5' |
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| 30965 | 3' | -52.8 | NC_006556.1 | + | 4992 | 0.67 | 0.4811 |
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Target: 5'- uGUACGACGCUagGCUgggGGCUuau-GGCGUc -3' miRNA: 3'- -CAUGCUGUGA--UGG---CCGGuuuuCCGCA- -5' |
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| 30965 | 3' | -52.8 | NC_006556.1 | + | 11467 | 0.69 | 0.366894 |
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Target: 5'- -aACGuAUAgUGCCGGCgAuAGGGCGUc -3' miRNA: 3'- caUGC-UGUgAUGGCCGgUuUUCCGCA- -5' |
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| 30965 | 3' | -52.8 | NC_006556.1 | + | 13268 | 1.08 | 0.000527 |
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Target: 5'- aGUACGACACUACCGGCCAAAAGGCGUa -3' miRNA: 3'- -CAUGCUGUGAUGGCCGGUUUUCCGCA- -5' |
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| 30965 | 3' | -52.8 | NC_006556.1 | + | 13350 | 0.7 | 0.321511 |
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Target: 5'- cUACGACGg-ACCcGCCAGAGGGCa- -3' miRNA: 3'- cAUGCUGUgaUGGcCGGUUUUCCGca -5' |
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| 30965 | 3' | -52.8 | NC_006556.1 | + | 18461 | 0.66 | 0.52676 |
|
Target: 5'- uUGCGGuCACUACUugaGGCCGuAAAGGaCGa -3' miRNA: 3'- cAUGCU-GUGAUGG---CCGGU-UUUCC-GCa -5' |
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| 30965 | 3' | -52.8 | NC_006556.1 | + | 19418 | 0.67 | 0.502564 |
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Target: 5'- cGUAUaGACGCcuuugguUGCCGGCCAGAuauucGGUGa -3' miRNA: 3'- -CAUG-CUGUG-------AUGGCCGGUUUu----CCGCa -5' |
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| 30965 | 3' | -52.8 | NC_006556.1 | + | 19590 | 0.69 | 0.396163 |
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Target: 5'- cUAUGuACGgUugCGGCCGAuuAGGGCGa -3' miRNA: 3'- cAUGC-UGUgAugGCCGGUU--UUCCGCa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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