Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30975 | 5' | -54.8 | NC_006557.1 | + | 30113 | 0.66 | 0.621631 |
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Target: 5'- aGUUGCGGCagugGuuGCAGCUGCUu- -3' miRNA: 3'- -CGACGUUGgugaCggCGUUGACGAca -5' |
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| 30975 | 5' | -54.8 | NC_006557.1 | + | 29705 | 0.66 | 0.610164 |
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Target: 5'- aGCUGUAAUUGCaGCCGCcACaccauucacuguUGCUGUu -3' miRNA: 3'- -CGACGUUGGUGaCGGCGuUG------------ACGACA- -5' |
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| 30975 | 5' | -54.8 | NC_006557.1 | + | 14341 | 0.66 | 0.587301 |
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Target: 5'- --aGCAACaggaggaUGCUGCAGCUGCUa- -3' miRNA: 3'- cgaCGUUGgug----ACGGCGUUGACGAca -5' |
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| 30975 | 5' | -54.8 | NC_006557.1 | + | 8834 | 0.67 | 0.51998 |
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Target: 5'- uGCUGUggUgGCUGCUGaUAGCcuUGCUGg -3' miRNA: 3'- -CGACGuuGgUGACGGC-GUUG--ACGACa -5' |
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| 30975 | 5' | -54.8 | NC_006557.1 | + | 2261 | 0.68 | 0.455897 |
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Target: 5'- uCUGCuuCUACUucaGCUGCAGCUGcCUGa -3' miRNA: 3'- cGACGuuGGUGA---CGGCGUUGAC-GACa -5' |
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| 30975 | 5' | -54.8 | NC_006557.1 | + | 30051 | 1.1 | 0.000495 |
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Target: 5'- aGCUGCAACCACUGCCGCAACUGCUGUa -3' miRNA: 3'- -CGACGUUGGUGACGGCGUUGACGACA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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