miRNA: 3'- gAAUG-GGAa--GUCCAC--UUUGUGCCUuc -5'
miRNA: 3'- gAAUGGGaaGUCCACuUUGUGCCuUC- -5'
miRNA: 3'- -GAAUGGGAAGUCCACUUUGUGCCUUC- -5'
Results 1 - 3 of 3 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30976 | 5' | -48.1 | NC_006558.1 | + | 3634 | 0.68 | 0.525362 |
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Target: 5'- aUUGCaCCUccgCGGGUGucAGGCGCGGAc- -3' miRNA: 3'- gAAUG-GGAa--GUCCAC--UUUGUGCCUuc -5' |
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| 30976 | 5' | -48.1 | NC_006558.1 | + | 889 | 0.7 | 0.407326 |
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Target: 5'- uUUACCUg-CAGGUGcGACACGGuGGu -3' miRNA: 3'- gAAUGGGaaGUCCACuUUGUGCCuUC- -5' |
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| 30976 | 5' | -48.1 | NC_006558.1 | + | 893 | 1.11 | 0.000546 |
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Target: 5'- uCUUACCCUUCAGGUGAAACACGGAAGg -3' miRNA: 3'- -GAAUGGGAAGUCCACUUUGUGCCUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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