Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30993 | 5' | -54.2 | NC_006560.1 | + | 38528 | 0.66 | 0.951396 |
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Target: 5'- -gGGGCcacaCCCAcggccgcgugcUCGAUCUCGGUCGu -3' miRNA: 3'- uaCCCGcca-GGGU-----------AGCUAGAGUUAGU- -5' |
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| 30993 | 5' | -54.2 | NC_006560.1 | + | 28066 | 0.66 | 0.937765 |
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Target: 5'- gGUGGGCGGUCCUGgggUGAg-UCGGUg- -3' miRNA: 3'- -UACCCGCCAGGGUa--GCUagAGUUAgu -5' |
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| 30993 | 5' | -54.2 | NC_006560.1 | + | 14217 | 0.66 | 0.937765 |
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Target: 5'- -cGGGCGGggcgCCCG-CGGUCgcggggaucgUCGAUCc -3' miRNA: 3'- uaCCCGCCa---GGGUaGCUAG----------AGUUAGu -5' |
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| 30993 | 5' | -54.2 | NC_006560.1 | + | 466 | 0.66 | 0.93169 |
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Target: 5'- -cGGGCGGccagucacccccgUCCCGgcccccgUCGGUCUCGcUCu -3' miRNA: 3'- uaCCCGCC-------------AGGGU-------AGCUAGAGUuAGu -5' |
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| 30993 | 5' | -54.2 | NC_006560.1 | + | 28104 | 0.7 | 0.783146 |
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Target: 5'- cGUGGGUGGUCCCGccugCGGUCcuggggUgAGUCGg -3' miRNA: 3'- -UACCCGCCAGGGUa---GCUAG------AgUUAGU- -5' |
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| 30993 | 5' | -54.2 | NC_006560.1 | + | 53768 | 0.71 | 0.724407 |
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Target: 5'- -gGGGCGG-CCCGUCGGUCgCGGc-- -3' miRNA: 3'- uaCCCGCCaGGGUAGCUAGaGUUagu -5' |
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| 30993 | 5' | -54.2 | NC_006560.1 | + | 148912 | 0.72 | 0.693785 |
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Target: 5'- cUGGGCGGgCgCAUCGAUUuuUUAAUCAg -3' miRNA: 3'- uACCCGCCaGgGUAGCUAG--AGUUAGU- -5' |
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| 30993 | 5' | -54.2 | NC_006560.1 | + | 45143 | 0.78 | 0.370704 |
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Target: 5'- -gGGGCGGUCCCGUCaGAUCcCGGUgGa -3' miRNA: 3'- uaCCCGCCAGGGUAG-CUAGaGUUAgU- -5' |
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| 30993 | 5' | -54.2 | NC_006560.1 | + | 148846 | 1.07 | 0.00503 |
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Target: 5'- gAUGGGCGGUCCCAUCGAUCUCAAUCAg -3' miRNA: 3'- -UACCCGCCAGGGUAGCUAGAGUUAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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