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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 310 | 3' | -56.4 | AC_000010.1 | + | 18175 | 0.66 | 0.493738 |
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Target: 5'- uUGGACUU-------GCCUCCUCCCCCu -3' miRNA: 3'- -ACUUGAGuacgaucUGGGGGAGGGGG- -5' |
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| 310 | 3' | -56.4 | AC_000010.1 | + | 10286 | 0.67 | 0.484256 |
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Target: 5'- ---cCUCAUGCUggaAGACCUCgCUCcuggcgCCCCg -3' miRNA: 3'- acuuGAGUACGA---UCUGGGG-GAG------GGGG- -5' |
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| 310 | 3' | -56.4 | AC_000010.1 | + | 6411 | 0.67 | 0.463507 |
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Target: 5'- -cGACUC-UGCUGGACCaaCgagCCCCUc -3' miRNA: 3'- acUUGAGuACGAUCUGGggGa--GGGGG- -5' |
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| 310 | 3' | -56.4 | AC_000010.1 | + | 7206 | 0.67 | 0.433285 |
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Target: 5'- cGAugUCAUuacAGGCCCCCUgUUCCCa -3' miRNA: 3'- aCUugAGUAcgaUCUGGGGGA-GGGGG- -5' |
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| 310 | 3' | -56.4 | AC_000010.1 | + | 13205 | 0.69 | 0.364824 |
|
Target: 5'- aUGAACUCugauuauuucaccaAUGCUAuacuGaACCCCCauuggcugCCCCCa -3' miRNA: 3'- -ACUUGAG--------------UACGAU----C-UGGGGGa-------GGGGG- -5' |
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| 310 | 3' | -56.4 | AC_000010.1 | + | 26184 | 0.74 | 0.17623 |
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Target: 5'- gGGACg-GUGCUGGGCCCCCcgaCCCg -3' miRNA: 3'- aCUUGagUACGAUCUGGGGGaggGGG- -5' |
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| 310 | 3' | -56.4 | AC_000010.1 | + | 6461 | 1.11 | 0.000297 |
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Target: 5'- aUGAACUCAUGCUAGACCCCCUCCCCCc -3' miRNA: 3'- -ACUUGAGUACGAUCUGGGGGAGGGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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