Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31000 | 3' | -51.2 | NC_006560.1 | + | 45274 | 0.66 | 0.995096 |
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Target: 5'- -gGGAGGUGCccCGggG-GUACgcgcgGAGGa -3' miRNA: 3'- ggCCUCCACGa-GUaaCaCAUGa----CUCC- -5' |
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| 31000 | 3' | -51.2 | NC_006560.1 | + | 116443 | 0.66 | 0.990074 |
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Target: 5'- cCCGGGGG-GCUCGcaGUGguaGaaGAGGa -3' miRNA: 3'- -GGCCUCCaCGAGUaaCACa--UgaCUCC- -5' |
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| 31000 | 3' | -51.2 | NC_006560.1 | + | 39913 | 0.66 | 0.990074 |
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Target: 5'- gCCGG-GGUGCgcucggGUGgcugggugGCUGGGGc -3' miRNA: 3'- -GGCCuCCACGaguaa-CACa-------UGACUCC- -5' |
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| 31000 | 3' | -51.2 | NC_006560.1 | + | 49685 | 0.67 | 0.98719 |
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Target: 5'- -aGGGGGUGgUgGgUGUGUAUaUGGGGg -3' miRNA: 3'- ggCCUCCACgAgUaACACAUG-ACUCC- -5' |
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| 31000 | 3' | -51.2 | NC_006560.1 | + | 145468 | 0.68 | 0.97953 |
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Target: 5'- cCCGGGGGggGCUCcgcccccUGUGUcccuGCgGGGGg -3' miRNA: 3'- -GGCCUCCa-CGAGua-----ACACA----UGaCUCC- -5' |
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| 31000 | 3' | -51.2 | NC_006560.1 | + | 34287 | 0.68 | 0.974618 |
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Target: 5'- aCGGGGGUGUgcgCGccugUGUGUcugcGCcGAGGg -3' miRNA: 3'- gGCCUCCACGa--GUa---ACACA----UGaCUCC- -5' |
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| 31000 | 3' | -51.2 | NC_006560.1 | + | 27836 | 0.74 | 0.771952 |
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Target: 5'- gCCGGGGGUGC-CGggG-GUGCcGGGGg -3' miRNA: 3'- -GGCCUCCACGaGUaaCaCAUGaCUCC- -5' |
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| 31000 | 3' | -51.2 | NC_006560.1 | + | 138018 | 1.14 | 0.004577 |
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Target: 5'- cCCGGAGGUGCUCAUUGUGUACUGAGGa -3' miRNA: 3'- -GGCCUCCACGAGUAACACAUGACUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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