Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31002 | 3' | -53.4 | NC_006560.1 | + | 138125 | 0.66 | 0.949193 |
Target: 5'- cGUCgGCCUGCGUGCU-----GCCGGAc -3' miRNA: 3'- -CAG-UGGAUGCACGAguuguCGGUCUa -5' |
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31002 | 3' | -53.4 | NC_006560.1 | + | 17095 | 0.66 | 0.939942 |
Target: 5'- cGUgACCUGCGcgagGCUCAGgGGgCGGGa -3' miRNA: 3'- -CAgUGGAUGCa---CGAGUUgUCgGUCUa -5' |
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31002 | 3' | -53.4 | NC_006560.1 | + | 3716 | 0.66 | 0.939942 |
Target: 5'- -gCACCagGCGcaGCUCGcGCAGCCAGGc -3' miRNA: 3'- caGUGGa-UGCa-CGAGU-UGUCGGUCUa -5' |
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31002 | 3' | -53.4 | NC_006560.1 | + | 39093 | 0.67 | 0.924148 |
Target: 5'- cUCGCUgACGcGCUCGGCGGCCGc-- -3' miRNA: 3'- cAGUGGaUGCaCGAGUUGUCGGUcua -5' |
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31002 | 3' | -53.4 | NC_006560.1 | + | 110755 | 0.67 | 0.912331 |
Target: 5'- -cCGCCUACGgGCacgCGGCcGCCAGGUc -3' miRNA: 3'- caGUGGAUGCaCGa--GUUGuCGGUCUA- -5' |
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31002 | 3' | -53.4 | NC_006560.1 | + | 51670 | 0.69 | 0.855126 |
Target: 5'- -aCGCgUGCGUGCUCcgGGCGGCCGc-- -3' miRNA: 3'- caGUGgAUGCACGAG--UUGUCGGUcua -5' |
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31002 | 3' | -53.4 | NC_006560.1 | + | 90415 | 0.69 | 0.846933 |
Target: 5'- aUCACCUucACGaUGCUCGACGGCgcccCGGGc -3' miRNA: 3'- cAGUGGA--UGC-ACGAGUUGUCG----GUCUa -5' |
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31002 | 3' | -53.4 | NC_006560.1 | + | 11665 | 0.69 | 0.846933 |
Target: 5'- aGUCGCUUcCGgggGCUCGGCGGuCCGGGc -3' miRNA: 3'- -CAGUGGAuGCa--CGAGUUGUC-GGUCUa -5' |
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31002 | 3' | -53.4 | NC_006560.1 | + | 11626 | 0.69 | 0.846933 |
Target: 5'- aGUCGCUUcCGgggGCUCGGCGGuCCGGGc -3' miRNA: 3'- -CAGUGGAuGCa--CGAGUUGUC-GGUCUa -5' |
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31002 | 3' | -53.4 | NC_006560.1 | + | 87794 | 0.72 | 0.703842 |
Target: 5'- gGUCGCC-GCGcccccGCUCGACGGUCAGGUu -3' miRNA: 3'- -CAGUGGaUGCa----CGAGUUGUCGGUCUA- -5' |
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31002 | 3' | -53.4 | NC_006560.1 | + | 111637 | 0.73 | 0.608693 |
Target: 5'- cGUCACCUACGgcgagauggUGCUCAGCGGCa---- -3' miRNA: 3'- -CAGUGGAUGC---------ACGAGUUGUCGgucua -5' |
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31002 | 3' | -53.4 | NC_006560.1 | + | 136879 | 1.05 | 0.00669 |
Target: 5'- cGUCACCUACGUGCUCAACAGCCAGAUc -3' miRNA: 3'- -CAGUGGAUGCACGAGUUGUCGGUCUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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