Results 1 - 20 of 20 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31004 | 3' | -55 | NC_006560.1 | + | 12951 | 0.66 | 0.929074 |
Target: 5'- cGGCcACGACGcGCUGGUuCUccGGAUGAa -3' miRNA: 3'- -UUGaUGCUGCaCGACCAcGA--CCUGCU- -5' |
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31004 | 3' | -55 | NC_006560.1 | + | 115106 | 0.66 | 0.917971 |
Target: 5'- cGACgaucCGGCGgcaGCUGGcGCUGGcCGAc -3' miRNA: 3'- -UUGau--GCUGCa--CGACCaCGACCuGCU- -5' |
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31004 | 3' | -55 | NC_006560.1 | + | 138593 | 0.66 | 0.917971 |
Target: 5'- aGACggggGCGGCG-GC-GGaccUGCUGGugGAg -3' miRNA: 3'- -UUGa---UGCUGCaCGaCC---ACGACCugCU- -5' |
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31004 | 3' | -55 | NC_006560.1 | + | 76671 | 0.66 | 0.917971 |
Target: 5'- ---gACGAgGUGUgGGUGCgcuucGGGCGAg -3' miRNA: 3'- uugaUGCUgCACGaCCACGa----CCUGCU- -5' |
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31004 | 3' | -55 | NC_006560.1 | + | 71625 | 0.66 | 0.917971 |
Target: 5'- aGGCcguCGACGgcUGcCUGGUGgaGGGCGGg -3' miRNA: 3'- -UUGau-GCUGC--AC-GACCACgaCCUGCU- -5' |
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31004 | 3' | -55 | NC_006560.1 | + | 25930 | 0.66 | 0.91205 |
Target: 5'- cGCggaGGCGUGC-GGUGCcggagggGGGCGAg -3' miRNA: 3'- uUGaugCUGCACGaCCACGa------CCUGCU- -5' |
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31004 | 3' | -55 | NC_006560.1 | + | 68374 | 0.66 | 0.905885 |
Target: 5'- -cCUGCG-CGacGC-GGUGCUGGACGc -3' miRNA: 3'- uuGAUGCuGCa-CGaCCACGACCUGCu -5' |
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31004 | 3' | -55 | NC_006560.1 | + | 99353 | 0.66 | 0.905885 |
Target: 5'- cACUGCGcCG-GCUGG-GCguacugGGGCGGg -3' miRNA: 3'- uUGAUGCuGCaCGACCaCGa-----CCUGCU- -5' |
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31004 | 3' | -55 | NC_006560.1 | + | 77660 | 0.67 | 0.899478 |
Target: 5'- cGCUGgGACGaGCUGG-GgUGGGCGc -3' miRNA: 3'- uUGAUgCUGCaCGACCaCgACCUGCu -5' |
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31004 | 3' | -55 | NC_006560.1 | + | 8535 | 0.67 | 0.878835 |
Target: 5'- cGCUGCG-CGUGgUGGggGUgGGGCGAc -3' miRNA: 3'- uUGAUGCuGCACgACCa-CGaCCUGCU- -5' |
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31004 | 3' | -55 | NC_006560.1 | + | 150227 | 0.68 | 0.863164 |
Target: 5'- cGCUGCcgGGCGUGCUGGUugcccgcGCUcucGGGCGc -3' miRNA: 3'- uUGAUG--CUGCACGACCA-------CGA---CCUGCu -5' |
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31004 | 3' | -55 | NC_006560.1 | + | 24399 | 0.68 | 0.839977 |
Target: 5'- gGACgUGCG-CGUGCUGGUGCUcuACGc -3' miRNA: 3'- -UUG-AUGCuGCACGACCACGAccUGCu -5' |
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31004 | 3' | -55 | NC_006560.1 | + | 146843 | 0.69 | 0.814271 |
Target: 5'- uGCUGCGGCG-GCgcgGG-GCgGGGCGGc -3' miRNA: 3'- uUGAUGCUGCaCGa--CCaCGaCCUGCU- -5' |
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31004 | 3' | -55 | NC_006560.1 | + | 74089 | 0.69 | 0.796266 |
Target: 5'- cGACcugGCGGCcugGCUGGcgucGCUGGGCGAc -3' miRNA: 3'- -UUGa--UGCUGca-CGACCa---CGACCUGCU- -5' |
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31004 | 3' | -55 | NC_006560.1 | + | 117457 | 0.7 | 0.738879 |
Target: 5'- cAGCUACGACGUGC-GGcGCgagcccGGGCGu -3' miRNA: 3'- -UUGAUGCUGCACGaCCaCGa-----CCUGCu -5' |
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31004 | 3' | -55 | NC_006560.1 | + | 91205 | 0.7 | 0.738879 |
Target: 5'- cGACUGCGACGUGCUGG-GCa------ -3' miRNA: 3'- -UUGAUGCUGCACGACCaCGaccugcu -5' |
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31004 | 3' | -55 | NC_006560.1 | + | 80853 | 0.71 | 0.708759 |
Target: 5'- gGGCUGCGcGCGgGCUGGgcgGCggGGGCGGc -3' miRNA: 3'- -UUGAUGC-UGCaCGACCa--CGa-CCUGCU- -5' |
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31004 | 3' | -55 | NC_006560.1 | + | 136248 | 0.73 | 0.553949 |
Target: 5'- gGGCUGCGAccCGUGCUGGUcuGCgUGGGCu- -3' miRNA: 3'- -UUGAUGCU--GCACGACCA--CG-ACCUGcu -5' |
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31004 | 3' | -55 | NC_006560.1 | + | 94957 | 0.75 | 0.484387 |
Target: 5'- cGCUGCGccGCGUGCUGGcGCgcaucggGGGCGGg -3' miRNA: 3'- uUGAUGC--UGCACGACCaCGa------CCUGCU- -5' |
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31004 | 3' | -55 | NC_006560.1 | + | 127980 | 1.07 | 0.00444 |
Target: 5'- cAACUACGACGUGCUGGUGCUGGACGAg -3' miRNA: 3'- -UUGAUGCUGCACGACCACGACCUGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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