Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31010 | 3' | -54.8 | NC_006560.1 | + | 107876 | 0.66 | 0.956021 |
Target: 5'- aGGUCacgCCCgucaGGCG-GG-CGACCCg -3' miRNA: 3'- -CCAGagaGGGaa--CUGCaCCaGCUGGG- -5' |
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31010 | 3' | -54.8 | NC_006560.1 | + | 143370 | 0.66 | 0.948009 |
Target: 5'- cGGUCUCgaCCCa-GGCGUaccgggGGgcggCGGCCCu -3' miRNA: 3'- -CCAGAGa-GGGaaCUGCA------CCa---GCUGGG- -5' |
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31010 | 3' | -54.8 | NC_006560.1 | + | 10568 | 0.66 | 0.948009 |
Target: 5'- cGGUCUCcagcaccugCCCgggGGgGUGGagggaGACCCa -3' miRNA: 3'- -CCAGAGa--------GGGaa-CUgCACCag---CUGGG- -5' |
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31010 | 3' | -54.8 | NC_006560.1 | + | 63213 | 0.66 | 0.945428 |
Target: 5'- cGGUUgagCUCCCggaacuccucggaGGCGaGGUCGAgCCCc -3' miRNA: 3'- -CCAGa--GAGGGaa-----------CUGCaCCAGCU-GGG- -5' |
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31010 | 3' | -54.8 | NC_006560.1 | + | 73264 | 0.66 | 0.943662 |
Target: 5'- uGGcCUCcacgcgcaugaCCCUcgccGACGUGG-CGGCCCa -3' miRNA: 3'- -CCaGAGa----------GGGAa---CUGCACCaGCUGGG- -5' |
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31010 | 3' | -54.8 | NC_006560.1 | + | 122554 | 0.67 | 0.939085 |
Target: 5'- cGGgUUCUCgCggUGGCGacugagccGGUCGGCCCg -3' miRNA: 3'- -CCaGAGAGgGa-ACUGCa-------CCAGCUGGG- -5' |
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31010 | 3' | -54.8 | NC_006560.1 | + | 32656 | 0.67 | 0.934275 |
Target: 5'- aGGcCg--CCCggcGGCGgcacggGGUCGGCCCa -3' miRNA: 3'- -CCaGagaGGGaa-CUGCa-----CCAGCUGGG- -5' |
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31010 | 3' | -54.8 | NC_006560.1 | + | 45915 | 0.68 | 0.9127 |
Target: 5'- gGGUCUC-CCCgggGGCGaGGacgaCGGCCUg -3' miRNA: 3'- -CCAGAGaGGGaa-CUGCaCCa---GCUGGG- -5' |
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31010 | 3' | -54.8 | NC_006560.1 | + | 105080 | 0.68 | 0.9127 |
Target: 5'- gGGUCUCgccgUCCUc-GCGgGGcCGGCCCg -3' miRNA: 3'- -CCAGAGa---GGGAacUGCaCCaGCUGGG- -5' |
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31010 | 3' | -54.8 | NC_006560.1 | + | 101466 | 0.68 | 0.9127 |
Target: 5'- uGUCgccgCUCCggacGGCGUcgcGGUCGGCCCc -3' miRNA: 3'- cCAGa---GAGGgaa-CUGCA---CCAGCUGGG- -5' |
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31010 | 3' | -54.8 | NC_006560.1 | + | 56649 | 0.68 | 0.894083 |
Target: 5'- ---gUCUCCCggGugG-GG-CGACCCa -3' miRNA: 3'- ccagAGAGGGaaCugCaCCaGCUGGG- -5' |
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31010 | 3' | -54.8 | NC_006560.1 | + | 118628 | 0.69 | 0.85863 |
Target: 5'- uGGUCUCguacgagaaCUCggUGGCGUGGggCGGCCg -3' miRNA: 3'- -CCAGAGa--------GGGa-ACUGCACCa-GCUGGg -5' |
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31010 | 3' | -54.8 | NC_006560.1 | + | 145517 | 0.71 | 0.754866 |
Target: 5'- --gCUCUCCCg-GGCG-GGccUCGACCCg -3' miRNA: 3'- ccaGAGAGGGaaCUGCaCC--AGCUGGG- -5' |
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31010 | 3' | -54.8 | NC_006560.1 | + | 120871 | 1.15 | 0.002004 |
Target: 5'- uGGUCUCUCCCUUGACGUGGUCGACCCg -3' miRNA: 3'- -CCAGAGAGGGAACUGCACCAGCUGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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