Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31012 | 5' | -57.9 | NC_006560.1 | + | 86320 | 0.7 | 0.533179 |
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Target: 5'- gGUCGCCCAGCGCGcGUGC-ACGu--- -3' miRNA: 3'- -UAGUGGGUCGCGCaCGCGaUGCucaa -5' |
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| 31012 | 5' | -57.9 | NC_006560.1 | + | 2255 | 0.71 | 0.51336 |
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Target: 5'- ---cCCCAGCGCGcgcagGCGCggUGCGAGUg -3' miRNA: 3'- uaguGGGUCGCGCa----CGCG--AUGCUCAa -5' |
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| 31012 | 5' | -57.9 | NC_006560.1 | + | 105848 | 0.71 | 0.493849 |
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Target: 5'- -gCGCUCGuGCGCGcGCGCUACGGGc- -3' miRNA: 3'- uaGUGGGU-CGCGCaCGCGAUGCUCaa -5' |
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| 31012 | 5' | -57.9 | NC_006560.1 | + | 115061 | 0.72 | 0.446657 |
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Target: 5'- -aCGCCgCGGCGCugcaGCGCUGCGAGg- -3' miRNA: 3'- uaGUGG-GUCGCGca--CGCGAUGCUCaa -5' |
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| 31012 | 5' | -57.9 | NC_006560.1 | + | 22902 | 0.73 | 0.368524 |
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Target: 5'- -gCGCCCgagguggcggaGGCGCGccgGCGCUACGAGg- -3' miRNA: 3'- uaGUGGG-----------UCGCGCa--CGCGAUGCUCaa -5' |
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| 31012 | 5' | -57.9 | NC_006560.1 | + | 125088 | 0.77 | 0.236404 |
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Target: 5'- gAUCGCCCGGCccgGCGUGCGCUAcuucgcccCGGGUc -3' miRNA: 3'- -UAGUGGGUCG---CGCACGCGAU--------GCUCAa -5' |
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| 31012 | 5' | -57.9 | NC_006560.1 | + | 118940 | 1.03 | 0.003999 |
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Target: 5'- cAUCACCCAGCGCGUGCGCUACGAGUUc -3' miRNA: 3'- -UAGUGGGUCGCGCACGCGAUGCUCAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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