Results 41 - 45 of 45 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio![]() |
P value |
Predicted miRNA align pattern | |||||||
31021 | 5' | -56.4 | NC_006560.1 | + | 62856 | 0.66 | 0.857882 |
Target: 5'- -cCGGAGCUccCGCaGCaGCGCGU-GUUGg -3' miRNA: 3'- acGCCUCGA--GCGaCG-CGUGCAuCAAC- -5' |
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31021 | 5' | -56.4 | NC_006560.1 | + | 112817 | 0.66 | 0.857882 |
Target: 5'- cGUGGAggcGCUCGC-GCaGCugGUGGgcGa -3' miRNA: 3'- aCGCCU---CGAGCGaCG-CGugCAUCaaC- -5' |
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31021 | 5' | -56.4 | NC_006560.1 | + | 3674 | 0.66 | 0.857882 |
Target: 5'- cGCGGAGgUCGCcGCGCAgGc----- -3' miRNA: 3'- aCGCCUCgAGCGaCGCGUgCaucaac -5' |
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31021 | 5' | -56.4 | NC_006560.1 | + | 101810 | 0.66 | 0.857882 |
Target: 5'- gGCGGAGCagCGCcGCGacCACGgcGUg- -3' miRNA: 3'- aCGCCUCGa-GCGaCGC--GUGCauCAac -5' |
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31021 | 5' | -56.4 | NC_006560.1 | + | 136157 | 0.65 | 0.891992 |
Target: 5'- gGCGGGGCUCcugggucgccaccuGCUGacgGC-CGUGGUg- -3' miRNA: 3'- aCGCCUCGAG--------------CGACg--CGuGCAUCAac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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