Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31026 | 5' | -53.5 | NC_006560.1 | + | 3728 | 0.66 | 0.959088 |
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Target: 5'- gCUCGCGcaGCCAGGcgcGCGggCGCGg- -3' miRNA: 3'- -GAGCGCa-UGGUCUaa-CGCaaGCGCga -5' |
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| 31026 | 5' | -53.5 | NC_006560.1 | + | 131132 | 0.66 | 0.959088 |
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Target: 5'- -cCGCG-GCCGGg--GCGcgCGCGCUc -3' miRNA: 3'- gaGCGCaUGGUCuaaCGCaaGCGCGA- -5' |
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| 31026 | 5' | -53.5 | NC_006560.1 | + | 126430 | 0.66 | 0.962675 |
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Target: 5'- -gCGCGcGCCGGAUcGCcGagCGCGCg -3' miRNA: 3'- gaGCGCaUGGUCUAaCG-CaaGCGCGa -5' |
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| 31026 | 5' | -53.5 | NC_006560.1 | + | 92657 | 0.66 | 0.962675 |
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Target: 5'- -cCGCGgGCCGGGUU-CGUgUGCGCg -3' miRNA: 3'- gaGCGCaUGGUCUAAcGCAaGCGCGa -5' |
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| 31026 | 5' | -53.5 | NC_006560.1 | + | 41264 | 0.66 | 0.968569 |
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Target: 5'- cCUCGCGcccacgcucaggGCCAGGaacguccggGCGgUCGCGCg -3' miRNA: 3'- -GAGCGCa-----------UGGUCUaa-------CGCaAGCGCGa -5' |
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| 31026 | 5' | -53.5 | NC_006560.1 | + | 33845 | 0.66 | 0.969181 |
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Target: 5'- -cCGCGgGCguGGggGCGcgCGCGCg -3' miRNA: 3'- gaGCGCaUGguCUaaCGCaaGCGCGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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