Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31039 | 3' | -50.3 | NC_006560.1 | + | 136187 | 0.71 | 0.932269 |
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Target: 5'- gGCCgugGUGUACUGCu-GGugGaUGACCa -3' miRNA: 3'- gUGG---UACAUGAUGuuCCugC-ACUGGc -5' |
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| 31039 | 3' | -50.3 | NC_006560.1 | + | 9708 | 0.71 | 0.921244 |
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Target: 5'- uCGCCGcgGUGCUgACGAcGACGUGGCCc -3' miRNA: 3'- -GUGGUa-CAUGA-UGUUcCUGCACUGGc -5' |
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| 31039 | 3' | -50.3 | NC_006560.1 | + | 135945 | 0.71 | 0.921244 |
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Target: 5'- gACCugacGUACUACuGGGAgGUGAUCGc -3' miRNA: 3'- gUGGua--CAUGAUGuUCCUgCACUGGC- -5' |
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| 31039 | 3' | -50.3 | NC_006560.1 | + | 107754 | 0.72 | 0.902788 |
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Target: 5'- gCACCAgGUGCgcgGCGAGGAgCGcGGCCa -3' miRNA: 3'- -GUGGUaCAUGa--UGUUCCU-GCaCUGGc -5' |
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| 31039 | 3' | -50.3 | NC_006560.1 | + | 130092 | 0.74 | 0.82573 |
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Target: 5'- gCGCCAUGg---GCGAGGACG-GGCUGg -3' miRNA: 3'- -GUGGUACaugaUGUUCCUGCaCUGGC- -5' |
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| 31039 | 3' | -50.3 | NC_006560.1 | + | 89736 | 0.74 | 0.80781 |
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Target: 5'- uCACCGUGgagagcgGCUuCGAGGccaACGUGGCCGu -3' miRNA: 3'- -GUGGUACa------UGAuGUUCC---UGCACUGGC- -5' |
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| 31039 | 3' | -50.3 | NC_006560.1 | + | 110126 | 0.77 | 0.678203 |
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Target: 5'- gGCgAUGUACUACGGGGAgaCG-GGCCGg -3' miRNA: 3'- gUGgUACAUGAUGUUCCU--GCaCUGGC- -5' |
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| 31039 | 3' | -50.3 | NC_006560.1 | + | 99402 | 0.78 | 0.625308 |
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Target: 5'- gCGCCAgcuccUGCUGCAAGGACGUcACCGc -3' miRNA: 3'- -GUGGUac---AUGAUGUUCCUGCAcUGGC- -5' |
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| 31039 | 3' | -50.3 | NC_006560.1 | + | 9044 | 0.79 | 0.583032 |
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Target: 5'- uCACCAgGUAC-ACGGGGGCGUcGACCGc -3' miRNA: 3'- -GUGGUaCAUGaUGUUCCUGCA-CUGGC- -5' |
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| 31039 | 3' | -50.3 | NC_006560.1 | + | 96444 | 1.09 | 0.010574 |
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Target: 5'- cCACCAUGUACUACAAGGACGUGACCGu -3' miRNA: 3'- -GUGGUACAUGAUGUUCCUGCACUGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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