Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31039 | 5' | -56.4 | NC_006560.1 | + | 12880 | 0.66 | 0.871494 |
Target: 5'- cUCGGGCgggaGCagGGUGCUCGUGG-UCGg -3' miRNA: 3'- aAGCCCG----UGg-CCAUGAGCGUCaAGUa -5' |
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31039 | 5' | -56.4 | NC_006560.1 | + | 67029 | 0.66 | 0.863164 |
Target: 5'- aUCGGGCcgucgcggcgagcGCCGGUggGC-CGCAGgaCGUg -3' miRNA: 3'- aAGCCCG-------------UGGCCA--UGaGCGUCaaGUA- -5' |
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31039 | 5' | -56.4 | NC_006560.1 | + | 11503 | 0.67 | 0.83497 |
Target: 5'- cUCGGGCGCCGGggaggcGCUCGUcgcccgacccccgccGGgggUCGc -3' miRNA: 3'- aAGCCCGUGGCCa-----UGAGCG---------------UCa--AGUa -5' |
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31039 | 5' | -56.4 | NC_006560.1 | + | 80524 | 0.67 | 0.787029 |
Target: 5'- -gCGGaGCACCGGUcgcagcgcGCUgGCGGUccUCAUc -3' miRNA: 3'- aaGCC-CGUGGCCA--------UGAgCGUCA--AGUA- -5' |
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31039 | 5' | -56.4 | NC_006560.1 | + | 147954 | 0.68 | 0.738879 |
Target: 5'- -gCGGGC-CCGG-GCUCGCAGagcUCGg -3' miRNA: 3'- aaGCCCGuGGCCaUGAGCGUCa--AGUa -5' |
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31039 | 5' | -56.4 | NC_006560.1 | + | 18958 | 0.68 | 0.728922 |
Target: 5'- -gCGGGCGCCGG-GCgUCGCGGgccgCGa -3' miRNA: 3'- aaGCCCGUGGCCaUG-AGCGUCaa--GUa -5' |
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31039 | 5' | -56.4 | NC_006560.1 | + | 106007 | 0.7 | 0.636566 |
Target: 5'- -cCGGGCAagCGGUAC-CGCAcGUUCGUc -3' miRNA: 3'- aaGCCCGUg-GCCAUGaGCGU-CAAGUA- -5' |
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31039 | 5' | -56.4 | NC_006560.1 | + | 51795 | 0.71 | 0.574408 |
Target: 5'- -gCGGGCGCCGGgaccgugGCccCGCGGUUCc- -3' miRNA: 3'- aaGCCCGUGGCCa------UGa-GCGUCAAGua -5' |
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31039 | 5' | -56.4 | NC_006560.1 | + | 31122 | 0.75 | 0.376161 |
Target: 5'- cUCGGGCGCCGGgggcgccuccgGCUCGCGGg---- -3' miRNA: 3'- aAGCCCGUGGCCa----------UGAGCGUCaagua -5' |
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31039 | 5' | -56.4 | NC_006560.1 | + | 43284 | 0.75 | 0.376161 |
Target: 5'- cUUCaGGGCGCCGGU-CUCGCGGacgUCGg -3' miRNA: 3'- -AAG-CCCGUGGCCAuGAGCGUCa--AGUa -5' |
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31039 | 5' | -56.4 | NC_006560.1 | + | 96484 | 1.04 | 0.004698 |
Target: 5'- gUUCGGGCACCGGUACUCGCAGUUCAUg -3' miRNA: 3'- -AAGCCCGUGGCCAUGAGCGUCAAGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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