Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 3104 | 3' | -51.9 | NC_001493.1 | + | 36236 | 0.66 | 0.975912 |
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Target: 5'- gGGUgCucaGUGggGCGGGUugCGCGGACGAu -3' miRNA: 3'- -UCA-Gug-UACuaUGUCCA--GCGCCUGUU- -5' |
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| 3104 | 3' | -51.9 | NC_001493.1 | + | 73324 | 0.66 | 0.967076 |
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Target: 5'- -aUUACAaGAUGgGGGcCGUGGACAGg -3' miRNA: 3'- ucAGUGUaCUAUgUCCaGCGCCUGUU- -5' |
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| 3104 | 3' | -51.9 | NC_001493.1 | + | 130971 | 0.66 | 0.967076 |
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Target: 5'- cGGUCACcccuUGCGGGUUcCGGACGGg -3' miRNA: 3'- -UCAGUGuacuAUGUCCAGcGCCUGUU- -5' |
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| 3104 | 3' | -51.9 | NC_001493.1 | + | 15417 | 0.66 | 0.967076 |
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Target: 5'- cGGUCACcccuUGCGGGUUcCGGACGGg -3' miRNA: 3'- -UCAGUGuacuAUGUCCAGcGCCUGUU- -5' |
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| 3104 | 3' | -51.9 | NC_001493.1 | + | 39097 | 0.67 | 0.960042 |
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Target: 5'- cGGUCGCGUG-UACGGGguuaccgGCGGAUu- -3' miRNA: 3'- -UCAGUGUACuAUGUCCag-----CGCCUGuu -5' |
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| 3104 | 3' | -51.9 | NC_001493.1 | + | 87549 | 0.67 | 0.952036 |
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Target: 5'- -----gGUGGUGCcGGUCGCGGGCu- -3' miRNA: 3'- ucagugUACUAUGuCCAGCGCCUGuu -5' |
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| 3104 | 3' | -51.9 | NC_001493.1 | + | 66330 | 0.69 | 0.9097 |
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Target: 5'- cGUCACuaca-ACAGGUCGUGcGACAGa -3' miRNA: 3'- uCAGUGuacuaUGUCCAGCGC-CUGUU- -5' |
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| 3104 | 3' | -51.9 | NC_001493.1 | + | 37638 | 0.69 | 0.903231 |
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Target: 5'- --cCAaacaAUGGUACAGGUCGCuccGGGCAc -3' miRNA: 3'- ucaGUg---UACUAUGUCCAGCG---CCUGUu -5' |
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| 3104 | 3' | -51.9 | NC_001493.1 | + | 12972 | 0.71 | 0.825193 |
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Target: 5'- gAGUCGcCAUcGGUuc-GGUCGCGGACAGg -3' miRNA: 3'- -UCAGU-GUA-CUAuguCCAGCGCCUGUU- -5' |
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| 3104 | 3' | -51.9 | NC_001493.1 | + | 93994 | 1.06 | 0.007835 |
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Target: 5'- gAGUCACAUGAUACAGGUCGCGGACAAu -3' miRNA: 3'- -UCAGUGUACUAUGUCCAGCGCCUGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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