Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31056 | 3' | -57.3 | NC_006560.1 | + | 95261 | 0.66 | 0.826719 |
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Target: 5'- cGCCgugUCgaacGGCUUCGC-GGCgcgcGUGCg -3' miRNA: 3'- -CGGaa-AGa---CCGGAGCGaCCGa---CACGa -5' |
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| 31056 | 3' | -57.3 | NC_006560.1 | + | 73350 | 0.67 | 0.763316 |
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Target: 5'- cGCCUggcgCUGGCCaaGCUGGUUcucGUGg- -3' miRNA: 3'- -CGGAaa--GACCGGagCGACCGA---CACga -5' |
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| 31056 | 3' | -57.3 | NC_006560.1 | + | 74098 | 0.68 | 0.694158 |
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Target: 5'- gGCCUggCUGGCgUCGCUgGGCgacGCc -3' miRNA: 3'- -CGGAaaGACCGgAGCGA-CCGacaCGa -5' |
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| 31056 | 3' | -57.3 | NC_006560.1 | + | 94020 | 0.68 | 0.692129 |
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Target: 5'- gGCCUucUUCgcggaguccaugGGCCUCGCgcGGCcGUGCc -3' miRNA: 3'- -CGGA--AAGa-----------CCGGAGCGa-CCGaCACGa -5' |
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| 31056 | 3' | -57.3 | NC_006560.1 | + | 70654 | 0.73 | 0.454063 |
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Target: 5'- gGCaCUUUCUGGCg-CGCUGGCggcgGCUg -3' miRNA: 3'- -CG-GAAAGACCGgaGCGACCGaca-CGA- -5' |
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| 31056 | 3' | -57.3 | NC_006560.1 | + | 51170 | 0.75 | 0.334972 |
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Target: 5'- cGCCUUUCUGGggcgcguCCUCGacgucCUGGCgGUGCUc -3' miRNA: 3'- -CGGAAAGACC-------GGAGC-----GACCGaCACGA- -5' |
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| 31056 | 3' | -57.3 | NC_006560.1 | + | 24266 | 0.75 | 0.328191 |
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Target: 5'- cGCgCgcgCUGGCCUCGCUGGCcGcGCg -3' miRNA: 3'- -CG-GaaaGACCGGAGCGACCGaCaCGa -5' |
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| 31056 | 3' | -57.3 | NC_006560.1 | + | 84787 | 1.1 | 0.001475 |
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Target: 5'- cGCCUUUCUGGCCUCGCUGGCUGUGCUg -3' miRNA: 3'- -CGGAAAGACCGGAGCGACCGACACGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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