Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 3106 | 5' | -53 | NC_001493.1 | + | 21634 | 0.66 | 0.978091 |
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Target: 5'- uGGGuUGUUGCaguGAUCGU---UCUCCGg -3' miRNA: 3'- cCCCuACAACG---CUAGCAcugAGAGGC- -5' |
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| 3106 | 5' | -53 | NC_001493.1 | + | 52093 | 0.66 | 0.970112 |
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Target: 5'- gGGGGGUGUUcCGggUGUaGCUCUCa- -3' miRNA: 3'- -CCCCUACAAcGCuaGCAcUGAGAGgc -5' |
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| 3106 | 5' | -53 | NC_001493.1 | + | 37543 | 0.66 | 0.967041 |
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Target: 5'- cGGGGAUGgaGgGGUCGUcGACcgggUCgcCCGg -3' miRNA: 3'- -CCCCUACaaCgCUAGCA-CUG----AGa-GGC- -5' |
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| 3106 | 5' | -53 | NC_001493.1 | + | 61509 | 0.67 | 0.956506 |
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Target: 5'- gGGGGAUGgacUG-GGUCGcGGgaCUCCGg -3' miRNA: 3'- -CCCCUACa--ACgCUAGCaCUgaGAGGC- -5' |
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| 3106 | 5' | -53 | NC_001493.1 | + | 126404 | 0.68 | 0.934259 |
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Target: 5'- cGGccgGUUcGCGAUCcggGACUCUCCGg -3' miRNA: 3'- cCCcuaCAA-CGCUAGca-CUGAGAGGC- -5' |
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| 3106 | 5' | -53 | NC_001493.1 | + | 10850 | 0.68 | 0.934259 |
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Target: 5'- cGGccgGUUcGCGAUCcggGACUCUCCGg -3' miRNA: 3'- cCCcuaCAA-CGCUAGca-CUGAGAGGC- -5' |
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| 3106 | 5' | -53 | NC_001493.1 | + | 50595 | 0.69 | 0.91206 |
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Target: 5'- uGGccGAUGgugUGCGGUCGUGGgcaUCUCUGg -3' miRNA: 3'- cCC--CUACa--ACGCUAGCACUg--AGAGGC- -5' |
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| 3106 | 5' | -53 | NC_001493.1 | + | 36866 | 0.69 | 0.899491 |
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Target: 5'- cGGGccGUUGUGGUCGcUGGCggUCCGc -3' miRNA: 3'- cCCCuaCAACGCUAGC-ACUGagAGGC- -5' |
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| 3106 | 5' | -53 | NC_001493.1 | + | 95697 | 1.11 | 0.003973 |
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Target: 5'- cGGGGAUGUUGCGAUCGUGACUCUCCGu -3' miRNA: 3'- -CCCCUACAACGCUAGCACUGAGAGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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