Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 3108 | 5' | -48.8 | NC_001493.1 | + | 48604 | 0.66 | 0.998573 |
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Target: 5'- uCUUGguguUGGCCC--GGAAGAcgUGCUCUCc -3' miRNA: 3'- -GAGC----AUCGGGuaUCUUCU--AUGAGAGc -5' |
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| 3108 | 5' | -48.8 | NC_001493.1 | + | 83762 | 0.66 | 0.998307 |
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Target: 5'- cCUCGUGGCUCAUGGAgucguccaucaggaGGAUcggaACgugcgggagugcguuUCUCGg -3' miRNA: 3'- -GAGCAUCGGGUAUCU--------------UCUA----UG---------------AGAGC- -5' |
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| 3108 | 5' | -48.8 | NC_001493.1 | + | 103220 | 0.68 | 0.991252 |
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Target: 5'- gCUCGUAGCUCGUcc--GGUGCUCcgUCGg -3' miRNA: 3'- -GAGCAUCGGGUAucuuCUAUGAG--AGC- -5' |
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| 3108 | 5' | -48.8 | NC_001493.1 | + | 2068 | 0.69 | 0.986938 |
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Target: 5'- cCUCGUGGCCacuccucAGGGUGCUCUUu -3' miRNA: 3'- -GAGCAUCGGguauc--UUCUAUGAGAGc -5' |
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| 3108 | 5' | -48.8 | NC_001493.1 | + | 117623 | 0.69 | 0.986938 |
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Target: 5'- cCUCGUGGCCacuccucAGGGUGCUCUUu -3' miRNA: 3'- -GAGCAUCGGguauc--UUCUAUGAGAGc -5' |
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| 3108 | 5' | -48.8 | NC_001493.1 | + | 18192 | 0.69 | 0.983259 |
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Target: 5'- -gCGU-GCCCAUGGAGGuGUACgCUUGg -3' miRNA: 3'- gaGCAuCGGGUAUCUUC-UAUGaGAGC- -5' |
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| 3108 | 5' | -48.8 | NC_001493.1 | + | 133746 | 0.69 | 0.983259 |
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Target: 5'- -gCGU-GCCCAUGGAGGuGUACgCUUGg -3' miRNA: 3'- gaGCAuCGGGUAUCUUC-UAUGaGAGC- -5' |
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| 3108 | 5' | -48.8 | NC_001493.1 | + | 21040 | 0.7 | 0.970688 |
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Target: 5'- aCUCGgGGCCCAcacgagAGAGGAUcUUCUUGa -3' miRNA: 3'- -GAGCaUCGGGUa-----UCUUCUAuGAGAGC- -5' |
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| 3108 | 5' | -48.8 | NC_001493.1 | + | 94984 | 0.7 | 0.967525 |
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Target: 5'- -cCGUGGCCUAgAGAuaGGAUGUUCUCGc -3' miRNA: 3'- gaGCAUCGGGUaUCU--UCUAUGAGAGC- -5' |
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| 3108 | 5' | -48.8 | NC_001493.1 | + | 98046 | 1.11 | 0.009423 |
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Target: 5'- cCUCGUAGCCCAUAGAAGAUACUCUCGg -3' miRNA: 3'- -GAGCAUCGGGUAUCUUCUAUGAGAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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