Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 311 | 5' | -53.4 | AC_000010.1 | + | 5330 | 0.66 | 0.679808 |
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Target: 5'- cCAUGAGUucguaguUGAGUGcCUCGGCuGCGUg -3' miRNA: 3'- cGUGUUCGu------ACUUAC-GAGCCG-CGCGg -5' |
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| 311 | 5' | -53.4 | AC_000010.1 | + | 12571 | 0.67 | 0.645268 |
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Target: 5'- -uGCAGGC-UGggUGCUgaggaaGGCGUucaacGCCa -3' miRNA: 3'- cgUGUUCGuACuuACGAg-----CCGCG-----CGG- -5' |
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| 311 | 5' | -53.4 | AC_000010.1 | + | 15992 | 0.67 | 0.622155 |
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Target: 5'- cGCAUA-GCggGAGUaGCcCGGUGgCGCCu -3' miRNA: 3'- -CGUGUuCGuaCUUA-CGaGCCGC-GCGG- -5' |
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| 311 | 5' | -53.4 | AC_000010.1 | + | 9263 | 0.68 | 0.542105 |
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Target: 5'- gGCGgGGGCGgagGAGgaggggGCacgCGGCGaCGCCg -3' miRNA: 3'- -CGUgUUCGUa--CUUa-----CGa--GCCGC-GCGG- -5' |
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| 311 | 5' | -53.4 | AC_000010.1 | + | 15752 | 0.7 | 0.448047 |
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Target: 5'- cGCACAgggugcuguguagAGCGUuugcGAGcaccuccguacaucuUGCUUGGCGCGCg -3' miRNA: 3'- -CGUGU-------------UCGUA----CUU---------------ACGAGCCGCGCGg -5' |
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| 311 | 5' | -53.4 | AC_000010.1 | + | 17394 | 0.75 | 0.23155 |
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Target: 5'- uGUugGGGCGcgGGAUGCgacgccacaggcggCGGCGCGCUa -3' miRNA: 3'- -CGugUUCGUa-CUUACGa-------------GCCGCGCGG- -5' |
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| 311 | 5' | -53.4 | AC_000010.1 | + | 7989 | 1.15 | 0.000294 |
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Target: 5'- aGCACAAGCAUGAAUGCUCGGCGCGCCg -3' miRNA: 3'- -CGUGUUCGUACUUACGAGCCGCGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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