Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 3110 | 3' | -56.5 | NC_001493.1 | + | 30495 | 0.66 | 0.863956 |
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Target: 5'- -aCUCGGgGC-CCUGgucgGgGGGAUCGGGu -3' miRNA: 3'- uaGAGCC-CGcGGACa---CgUUCUAGCUC- -5' |
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| 3110 | 3' | -56.5 | NC_001493.1 | + | 87962 | 0.66 | 0.831627 |
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Target: 5'- --aUC-GGCGCCUGgaGCGAucGAUCGAGa -3' miRNA: 3'- uagAGcCCGCGGACa-CGUU--CUAGCUC- -5' |
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| 3110 | 3' | -56.5 | NC_001493.1 | + | 70576 | 0.67 | 0.796311 |
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Target: 5'- cAUCUgGGGCGauaCCUGUGUgcGAcaaacUCGAGc -3' miRNA: 3'- -UAGAgCCCGC---GGACACGuuCU-----AGCUC- -5' |
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| 3110 | 3' | -56.5 | NC_001493.1 | + | 60218 | 0.67 | 0.7777 |
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Target: 5'- cUCgCGGGCGCCgGUGgCuuGGUCGAc -3' miRNA: 3'- uAGaGCCCGCGGaCAC-GuuCUAGCUc -5' |
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| 3110 | 3' | -56.5 | NC_001493.1 | + | 90111 | 0.68 | 0.748799 |
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Target: 5'- uAUCUgGGGUGCCa---CGGGGUCGAGa -3' miRNA: 3'- -UAGAgCCCGCGGacacGUUCUAGCUC- -5' |
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| 3110 | 3' | -56.5 | NC_001493.1 | + | 42888 | 0.68 | 0.748799 |
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Target: 5'- -gCUCGGGUGUUgGUGCcgguugugagGGGGUCGGGg -3' miRNA: 3'- uaGAGCCCGCGGaCACG----------UUCUAGCUC- -5' |
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| 3110 | 3' | -56.5 | NC_001493.1 | + | 28129 | 0.69 | 0.70883 |
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Target: 5'- -gUUCGGGUGCCgcgcGUGCcccGAUCGAu -3' miRNA: 3'- uaGAGCCCGCGGa---CACGuu-CUAGCUc -5' |
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| 3110 | 3' | -56.5 | NC_001493.1 | + | 79418 | 0.81 | 0.165235 |
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Target: 5'- ---gCGGGaGCCUGUGCGGGGUCGAGa -3' miRNA: 3'- uagaGCCCgCGGACACGUUCUAGCUC- -5' |
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| 3110 | 3' | -56.5 | NC_001493.1 | + | 100194 | 1.07 | 0.002676 |
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Target: 5'- cAUCUCGGGCGCCUGUGCAAGAUCGAGa -3' miRNA: 3'- -UAGAGCCCGCGGACACGUUCUAGCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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