Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 3110 | 5' | -56.3 | NC_001493.1 | + | 53327 | 0.66 | 0.856456 |
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Target: 5'- --gUGGGUCcCGCACAauGcCUUUCUGGa -3' miRNA: 3'- uugACCCAGaGCGUGU--CuGAGAGGCC- -5' |
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| 3110 | 5' | -56.3 | NC_001493.1 | + | 99713 | 0.66 | 0.848547 |
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Target: 5'- -uUUGGGUCacUGCcCGGACUCUCUGu -3' miRNA: 3'- uuGACCCAGa-GCGuGUCUGAGAGGCc -5' |
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| 3110 | 5' | -56.3 | NC_001493.1 | + | 21635 | 0.67 | 0.806165 |
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Target: 5'- --aUGGGUUgUUGCAguGAUcguUCUCCGGg -3' miRNA: 3'- uugACCCAG-AGCGUguCUG---AGAGGCC- -5' |
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| 3110 | 5' | -56.3 | NC_001493.1 | + | 109356 | 0.68 | 0.788035 |
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Target: 5'- cACUGGGgaUCGCGCccgcGCUCUcCCGGg -3' miRNA: 3'- uUGACCCagAGCGUGuc--UGAGA-GGCC- -5' |
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| 3110 | 5' | -56.3 | NC_001493.1 | + | 58747 | 0.68 | 0.747224 |
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Target: 5'- uAACUGGGUC-CaGCACGGGgaguugggcucgagUUCUCCGa -3' miRNA: 3'- -UUGACCCAGaG-CGUGUCU--------------GAGAGGCc -5' |
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| 3110 | 5' | -56.3 | NC_001493.1 | + | 94399 | 0.69 | 0.740381 |
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Target: 5'- uAACUGGGUCgucaUCGCGgAGAacagCCGGa -3' miRNA: 3'- -UUGACCCAG----AGCGUgUCUgagaGGCC- -5' |
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| 3110 | 5' | -56.3 | NC_001493.1 | + | 89980 | 0.69 | 0.690333 |
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Target: 5'- ---gGGGUCgCGCACGGACcggCCGGu -3' miRNA: 3'- uugaCCCAGaGCGUGUCUGagaGGCC- -5' |
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| 3110 | 5' | -56.3 | NC_001493.1 | + | 51617 | 0.69 | 0.690333 |
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Target: 5'- gAACaUGGGUCUCagauaGUACAGGC--UCCGGg -3' miRNA: 3'- -UUG-ACCCAGAG-----CGUGUCUGagAGGCC- -5' |
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| 3110 | 5' | -56.3 | NC_001493.1 | + | 49467 | 0.74 | 0.423137 |
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Target: 5'- ----cGGUCUCGCACcGACccUCUCCGGg -3' miRNA: 3'- uugacCCAGAGCGUGuCUG--AGAGGCC- -5' |
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| 3110 | 5' | -56.3 | NC_001493.1 | + | 100230 | 1.09 | 0.002463 |
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Target: 5'- gAACUGGGUCUCGCACAGACUCUCCGGa -3' miRNA: 3'- -UUGACCCAGAGCGUGUCUGAGAGGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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