Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31165 | 3' | -57.4 | NC_006560.1 | + | 12365 | 0.66 | 0.857135 |
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Target: 5'- gCGUCUCGaCGGgggggagaGGGCUCCGGUUc-- -3' miRNA: 3'- -GCAGAGC-GCCaa------CUCGAGGUCGAcca -5' |
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| 31165 | 3' | -57.4 | NC_006560.1 | + | 138377 | 0.66 | 0.857135 |
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Target: 5'- uCGaCUCGUGGgUGAGCUCCAcccGC-GGc -3' miRNA: 3'- -GCaGAGCGCCaACUCGAGGU---CGaCCa -5' |
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| 31165 | 3' | -57.4 | NC_006560.1 | + | 107106 | 0.67 | 0.807609 |
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Target: 5'- gGcC-CGCGGcgUGGGCUaUCGGCUGGUc -3' miRNA: 3'- gCaGaGCGCCa-ACUCGA-GGUCGACCA- -5' |
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| 31165 | 3' | -57.4 | NC_006560.1 | + | 101245 | 0.67 | 0.789759 |
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Target: 5'- gCGgg-UGCGGUugaUGAGCUCCAGCg--- -3' miRNA: 3'- -GCagaGCGCCA---ACUCGAGGUCGacca -5' |
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| 31165 | 3' | -57.4 | NC_006560.1 | + | 22323 | 0.68 | 0.742831 |
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Target: 5'- gCGgagCUCGCGGagcucgcgGAGCUCgCGGCgGGg -3' miRNA: 3'- -GCa--GAGCGCCaa------CUCGAG-GUCGaCCa -5' |
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| 31165 | 3' | -57.4 | NC_006560.1 | + | 73414 | 0.69 | 0.69351 |
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Target: 5'- aCGgcgagCUCGCGGagcugGAGCgCCAGCUGcGg -3' miRNA: 3'- -GCa----GAGCGCCaa---CUCGaGGUCGAC-Ca -5' |
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| 31165 | 3' | -57.4 | NC_006560.1 | + | 64897 | 0.7 | 0.622564 |
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Target: 5'- gCGuUCUCGCGaUUGAGCUCgAGCggcacgGGg -3' miRNA: 3'- -GC-AGAGCGCcAACUCGAGgUCGa-----CCa -5' |
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| 31165 | 3' | -57.4 | NC_006560.1 | + | 17445 | 0.71 | 0.571932 |
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Target: 5'- cCGcCUCGCGGUcGAggcGCUCCAGCaccuccggGGUg -3' miRNA: 3'- -GCaGAGCGCCAaCU---CGAGGUCGa-------CCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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