Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31185 | 3' | -58 | NC_006560.1 | + | 32748 | 0.71 | 0.504851 |
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Target: 5'- gGGGCCCGGCGcgccggccggggUCUCGggGUCCgaggGCGa -3' miRNA: 3'- -UUCGGGUCGC------------AGAGCuaCAGGag--CGC- -5' |
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| 31185 | 3' | -58 | NC_006560.1 | + | 62882 | 0.72 | 0.48577 |
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Target: 5'- gGGGCgCAGCGUCUCGccGUCg-CGCGu -3' miRNA: 3'- -UUCGgGUCGCAGAGCuaCAGgaGCGC- -5' |
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| 31185 | 3' | -58 | NC_006560.1 | + | 34699 | 0.72 | 0.457814 |
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Target: 5'- -cGCCgGGCGUCUCGGUGgcucUCUCGgGc -3' miRNA: 3'- uuCGGgUCGCAGAGCUACa---GGAGCgC- -5' |
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| 31185 | 3' | -58 | NC_006560.1 | + | 70826 | 0.72 | 0.448688 |
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Target: 5'- -cGCCgCGGCgGUC-CGGgagGUCCUCGCGg -3' miRNA: 3'- uuCGG-GUCG-CAGaGCUa--CAGGAGCGC- -5' |
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| 31185 | 3' | -58 | NC_006560.1 | + | 17347 | 0.75 | 0.34775 |
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Target: 5'- cGAGCCCGGCGUCagggcgCGGUGga-UCGCGa -3' miRNA: 3'- -UUCGGGUCGCAGa-----GCUACaggAGCGC- -5' |
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| 31185 | 3' | -58 | NC_006560.1 | + | 248 | 0.75 | 0.310753 |
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Target: 5'- cGGGCCCcaacuGCGUCUCGGUGgaggCCgggUCGCGa -3' miRNA: 3'- -UUCGGGu----CGCAGAGCUACa---GG---AGCGC- -5' |
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| 31185 | 3' | -58 | NC_006560.1 | + | 101867 | 0.76 | 0.303722 |
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Target: 5'- -cGCCCGcggccgcgaacuGCGUCUUGGgcagccUGUCCUCGCGg -3' miRNA: 3'- uuCGGGU------------CGCAGAGCU------ACAGGAGCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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