Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31198 | 3' | -56.9 | NC_006560.1 | + | 71317 | 0.66 | 0.866823 |
Target: 5'- cGCgucgUGUCGCGCcCGCUCGcGGGGg--- -3' miRNA: 3'- aCG----ACAGCGCGaGCGAGC-UCUCagau -5' |
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31198 | 3' | -56.9 | NC_006560.1 | + | 81044 | 0.66 | 0.826719 |
Target: 5'- cGCgg-CGCGCUCGCccagCGGGuGGUCg- -3' miRNA: 3'- aCGacaGCGCGAGCGa---GCUC-UCAGau -5' |
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31198 | 3' | -56.9 | NC_006560.1 | + | 5476 | 0.67 | 0.791377 |
Target: 5'- gGCUccgagCGCGgUCGCggCGAGGGUCg- -3' miRNA: 3'- aCGAca---GCGCgAGCGa-GCUCUCAGau -5' |
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31198 | 3' | -56.9 | NC_006560.1 | + | 89710 | 0.68 | 0.7728 |
Target: 5'- gGCgGUCGCGC-CGCUCGuc-GUCg- -3' miRNA: 3'- aCGaCAGCGCGaGCGAGCucuCAGau -5' |
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31198 | 3' | -56.9 | NC_006560.1 | + | 34754 | 0.7 | 0.642995 |
Target: 5'- cGC-GUCGC-CUCGCgagcUCGAGGGUCg- -3' miRNA: 3'- aCGaCAGCGcGAGCG----AGCUCUCAGau -5' |
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31198 | 3' | -56.9 | NC_006560.1 | + | 118487 | 0.71 | 0.561068 |
Target: 5'- aGCgccCGCGCUCGCUCGuGGAGUggCUGg -3' miRNA: 3'- aCGacaGCGCGAGCGAGC-UCUCA--GAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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