Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
312 | 5' | -60 | AC_000010.1 | + | 9356 | 0.66 | 0.365963 |
Target: 5'- gACCG---UUUGCCCGUGCGCcgccGGCGUc -3' miRNA: 3'- aUGGUcuuGGAUGGGCGCGCG----CCGCG- -5' |
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312 | 5' | -60 | AC_000010.1 | + | 17402 | 0.66 | 0.357353 |
Target: 5'- cGCgGGAugCgacGCCacagGCG-GCGGCGCg -3' miRNA: 3'- aUGgUCUugGa--UGGg---CGCgCGCCGCG- -5' |
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312 | 5' | -60 | AC_000010.1 | + | 5534 | 0.66 | 0.357353 |
Target: 5'- aACCAucuGcGCCU-CCUGCgGCGCGGaUGCa -3' miRNA: 3'- aUGGU---CuUGGAuGGGCG-CGCGCC-GCG- -5' |
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312 | 5' | -60 | AC_000010.1 | + | 9887 | 0.66 | 0.348887 |
Target: 5'- cGCguGAGCCcaaACCCGCGCaUGG-GCu -3' miRNA: 3'- aUGguCUUGGa--UGGGCGCGcGCCgCG- -5' |
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312 | 5' | -60 | AC_000010.1 | + | 16088 | 0.66 | 0.340564 |
Target: 5'- cUGCCAGcacuggcACCUGaagCCGCGCGUcuGGCuGCu -3' miRNA: 3'- -AUGGUCu------UGGAUg--GGCGCGCG--CCG-CG- -5' |
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312 | 5' | -60 | AC_000010.1 | + | 15392 | 0.66 | 0.324353 |
Target: 5'- aACCAGAuCCUcAUCCGCcCGCcaGCGCc -3' miRNA: 3'- aUGGUCUuGGA-UGGGCGcGCGc-CGCG- -5' |
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312 | 5' | -60 | AC_000010.1 | + | 8528 | 0.67 | 0.308723 |
Target: 5'- cGCCGGGGCCcgAgCCGCcucuacCGCcaGGCGCg -3' miRNA: 3'- aUGGUCUUGGa-UgGGCGc-----GCG--CCGCG- -5' |
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312 | 5' | -60 | AC_000010.1 | + | 12088 | 0.67 | 0.293675 |
Target: 5'- aGCCAccuCCUGauCCCgcaauGCGgGCGGCGCu -3' miRNA: 3'- aUGGUcuuGGAU--GGG-----CGCgCGCCGCG- -5' |
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312 | 5' | -60 | AC_000010.1 | + | 16151 | 0.7 | 0.198706 |
Target: 5'- gGCCAGcGCgUGCCCGUGCGCaccCGUc -3' miRNA: 3'- aUGGUCuUGgAUGGGCGCGCGcc-GCG- -5' |
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312 | 5' | -60 | AC_000010.1 | + | 12340 | 0.73 | 0.108075 |
Target: 5'- aGCCAGGACCUuCUCGUGgGUGGgGUu -3' miRNA: 3'- aUGGUCUUGGAuGGGCGCgCGCCgCG- -5' |
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312 | 5' | -60 | AC_000010.1 | + | 8578 | 1.08 | 0.000202 |
Target: 5'- gUACCAGAACCUACCCGCGCGCGGCGCc -3' miRNA: 3'- -AUGGUCUUGGAUGGGCGCGCGCCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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