Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31244 | 3' | -51.9 | NC_006560.1 | + | 15015 | 0.66 | 0.987022 |
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Target: 5'- gCAUCCUcGCcCGCGGGGUgcggcgUCGg -3' miRNA: 3'- -GUAGGA-CGaGCGCCUCGacauaaAGC- -5' |
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| 31244 | 3' | -51.9 | NC_006560.1 | + | 129683 | 0.66 | 0.981418 |
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Target: 5'- gAUCCUGUggGCcGAGCUGU---UCGg -3' miRNA: 3'- gUAGGACGagCGcCUCGACAuaaAGC- -5' |
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| 31244 | 3' | -51.9 | NC_006560.1 | + | 61011 | 0.67 | 0.971354 |
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Target: 5'- --aCCgaGCUCGUGGAGCUGa------ -3' miRNA: 3'- guaGGa-CGAGCGCCUCGACauaaagc -5' |
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| 31244 | 3' | -51.9 | NC_006560.1 | + | 137427 | 0.69 | 0.935623 |
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Target: 5'- aCAUCCUGCg---GGAGCUGU---UCGg -3' miRNA: 3'- -GUAGGACGagcgCCUCGACAuaaAGC- -5' |
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| 31244 | 3' | -51.9 | NC_006560.1 | + | 34031 | 0.69 | 0.924963 |
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Target: 5'- --gCCUGCUCGCGGgaGGuCUGgggAggUCGg -3' miRNA: 3'- guaGGACGAGCGCC--UC-GACa--UaaAGC- -5' |
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| 31244 | 3' | -51.9 | NC_006560.1 | + | 51193 | 0.7 | 0.907063 |
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Target: 5'- aCGUCCuggcggUGCUCGCGGAGCagaccguccagUGgcugUCGg -3' miRNA: 3'- -GUAGG------ACGAGCGCCUCG-----------ACauaaAGC- -5' |
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| 31244 | 3' | -51.9 | NC_006560.1 | + | 13204 | 0.7 | 0.907063 |
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Target: 5'- uCGUCCUcGCUCGUGGcGCUGaagcugUCGu -3' miRNA: 3'- -GUAGGA-CGAGCGCCuCGACauaa--AGC- -5' |
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| 31244 | 3' | -51.9 | NC_006560.1 | + | 73417 | 0.7 | 0.893873 |
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Target: 5'- --gCgaGCUCGCGGAGCUGgag--CGc -3' miRNA: 3'- guaGgaCGAGCGCCUCGACauaaaGC- -5' |
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| 31244 | 3' | -51.9 | NC_006560.1 | + | 50362 | 0.72 | 0.823046 |
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Target: 5'- cCAUCCgaucUGUUCGCGGccgAGCUGgcgUUCGa -3' miRNA: 3'- -GUAGG----ACGAGCGCC---UCGACauaAAGC- -5' |
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| 31244 | 3' | -51.9 | NC_006560.1 | + | 51292 | 0.74 | 0.685934 |
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Target: 5'- --cCCUGCUCGCcGAGCUGU--UUCa -3' miRNA: 3'- guaGGACGAGCGcCUCGACAuaAAGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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