Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
3125 | 3' | -57.4 | NC_001493.1 | + | 55086 | 0.7 | 0.60174 |
Target: 5'- aGCgAGCUCCGggcuggucggcuCAUcCGAGAacugugaugGUGGCGGCg -3' miRNA: 3'- -UGgUCGAGGC------------GUA-GCUCU---------CACCGUCG- -5' |
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3125 | 3' | -57.4 | NC_001493.1 | + | 90079 | 0.71 | 0.550878 |
Target: 5'- uACgAGCUCCcCca-GAGGGUGGUAGCc -3' miRNA: 3'- -UGgUCGAGGcGuagCUCUCACCGUCG- -5' |
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3125 | 3' | -57.4 | NC_001493.1 | + | 90992 | 0.74 | 0.367089 |
Target: 5'- cGCCGGCUCCGaGUCGAGAGgccGUucgAGCa -3' miRNA: 3'- -UGGUCGAGGCgUAGCUCUCac-CG---UCG- -5' |
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3125 | 3' | -57.4 | NC_001493.1 | + | 108919 | 0.66 | 0.826597 |
Target: 5'- cGCCcGCugUCCGCGUCGuGAGcaacGCGGUg -3' miRNA: 3'- -UGGuCG--AGGCGUAGCuCUCac--CGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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