Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 3125 | 5' | -53.6 | NC_001493.1 | + | 85946 | 0.72 | 0.715105 |
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Target: 5'- gGAGACGCaCCggaucucaacaUCGAUGAgcucCCGGACGg -3' miRNA: 3'- -CUUUGCG-GGag---------AGCUACUa---GGCCUGC- -5' |
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| 3125 | 5' | -53.6 | NC_001493.1 | + | 72560 | 0.66 | 0.96322 |
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Target: 5'- gGGuuCGUUUgCUCGAUGAaaucUCCGGACa -3' miRNA: 3'- -CUuuGCGGGaGAGCUACU----AGGCCUGc -5' |
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| 3125 | 5' | -53.6 | NC_001493.1 | + | 15214 | 0.66 | 0.96322 |
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Target: 5'- -uAACGCaCCacgagCUCGuagagGGUCCGGACc -3' miRNA: 3'- cuUUGCG-GGa----GAGCua---CUAGGCCUGc -5' |
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| 3125 | 5' | -53.6 | NC_001493.1 | + | 130768 | 0.66 | 0.96322 |
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Target: 5'- -uAACGCaCCacgagCUCGuagagGGUCCGGACc -3' miRNA: 3'- cuUUGCG-GGa----GAGCua---CUAGGCCUGc -5' |
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| 3125 | 5' | -53.6 | NC_001493.1 | + | 109257 | 0.66 | 0.959672 |
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Target: 5'- ----gGCCCUgaCGAUGuaCCGGGCGc -3' miRNA: 3'- cuuugCGGGAgaGCUACuaGGCCUGC- -5' |
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| 3125 | 5' | -53.6 | NC_001493.1 | + | 110498 | 0.67 | 0.938417 |
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Target: 5'- --cGCGCCCggagugaaaCUCGAUGGgccgCCGcGACa -3' miRNA: 3'- cuuUGCGGGa--------GAGCUACUa---GGC-CUGc -5' |
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| 3125 | 5' | -53.6 | NC_001493.1 | + | 49685 | 0.75 | 0.531877 |
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Target: 5'- uGAuAC-CCCUCUUGGUGGUCCuGACGg -3' miRNA: 3'- -CUuUGcGGGAGAGCUACUAGGcCUGC- -5' |
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| 3125 | 5' | -53.6 | NC_001493.1 | + | 131264 | 0.66 | 0.96557 |
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Target: 5'- ----aGCuCUUCUCGGUGAgcgcaucgagacccUCCGGGCc -3' miRNA: 3'- cuuugCG-GGAGAGCUACU--------------AGGCCUGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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