Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 3125 | 5' | -53.6 | NC_001493.1 | + | 128612 | 0.75 | 0.552103 |
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Target: 5'- cGggGuCGCCCUCUgGGUGAUCgUGGcACGg -3' miRNA: 3'- -CuuU-GCGGGAGAgCUACUAG-GCC-UGC- -5' |
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| 3125 | 5' | -53.6 | NC_001493.1 | + | 49685 | 0.75 | 0.531877 |
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Target: 5'- uGAuAC-CCCUCUUGGUGGUCCuGACGg -3' miRNA: 3'- -CUuUGcGGGAGAGCUACUAGGcCUGC- -5' |
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| 3125 | 5' | -53.6 | NC_001493.1 | + | 5827 | 1.04 | 0.009701 |
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Target: 5'- gGAAACGUCCUCUCGAUGAUCCGGACGa -3' miRNA: 3'- -CUUUGCGGGAGAGCUACUAGGCCUGC- -5' |
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| 3125 | 5' | -53.6 | NC_001493.1 | + | 5912 | 1.04 | 0.009701 |
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Target: 5'- gGAAACGUCCUCUCGAUGAUCCGGACGa -3' miRNA: 3'- -CUUUGCGGGAGAGCUACUAGGCCUGC- -5' |
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| 3125 | 5' | -53.6 | NC_001493.1 | + | 121381 | 1.04 | 0.009701 |
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Target: 5'- gGAAACGUCCUCUCGAUGAUCCGGACGa -3' miRNA: 3'- -CUUUGCGGGAGAGCUACUAGGCCUGC- -5' |
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| 3125 | 5' | -53.6 | NC_001493.1 | + | 121466 | 1.04 | 0.009701 |
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Target: 5'- gGAAACGUCCUCUCGAUGAUCCGGACGa -3' miRNA: 3'- -CUUUGCGGGAGAGCUACUAGGCCUGC- -5' |
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| 3125 | 5' | -53.6 | NC_001493.1 | + | 121267 | 1.09 | 0.004533 |
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Target: 5'- gGAAACGCCCUCUCGAUGAUCCGGACGa -3' miRNA: 3'- -CUUUGCGGGAGAGCUACUAGGCCUGC- -5' |
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| 3125 | 5' | -53.6 | NC_001493.1 | + | 5713 | 1.09 | 0.004533 |
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Target: 5'- gGAAACGCCCUCUCGAUGAUCCGGACGa -3' miRNA: 3'- -CUUUGCGGGAGAGCUACUAGGCCUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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