Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31255 | 3' | -55.6 | NC_006560.1 | + | 132573 | 0.72 | 0.648071 |
Target: 5'- --gUCUGGGUCUUGGGggCGU--CCGa -3' miRNA: 3'- gaaAGACCCGGAGCCCaaGCGaaGGC- -5' |
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31255 | 3' | -55.6 | NC_006560.1 | + | 11648 | 0.72 | 0.607151 |
Target: 5'- --gUCcGGGCgggCGGGggagUCGCUUCCGg -3' miRNA: 3'- gaaAGaCCCGga-GCCCa---AGCGAAGGC- -5' |
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31255 | 3' | -55.6 | NC_006560.1 | + | 98186 | 0.73 | 0.596949 |
Target: 5'- -cUUCgaGGGCCUCGGGgaCGUgggCCGc -3' miRNA: 3'- gaAAGa-CCCGGAGCCCaaGCGaa-GGC- -5' |
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31255 | 3' | -55.6 | NC_006560.1 | + | 783 | 1.1 | 0.00286 |
Target: 5'- cCUUUCUGGGCCUCGGGUUCGCUUCCGg -3' miRNA: 3'- -GAAAGACCCGGAGCCCAAGCGAAGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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