Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31283 | 3' | -46.5 | NC_006565.1 | + | 13302 | 0.66 | 0.999819 |
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Target: 5'- uAGACUUUAGAUuUCUaucuuuagcAGCUAGcGCCAc -3' miRNA: 3'- -UCUGAAAUUUGcGGA---------UCGAUCaUGGU- -5' |
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| 31283 | 3' | -46.5 | NC_006565.1 | + | 52729 | 0.66 | 0.999819 |
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Target: 5'- gAGACa--GAGCGCCUAGUaugggacuaGGUACUAu -3' miRNA: 3'- -UCUGaaaUUUGCGGAUCGa--------UCAUGGU- -5' |
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| 31283 | 3' | -46.5 | NC_006565.1 | + | 110562 | 0.66 | 0.999766 |
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Target: 5'- aAGACUau--ACGCCUGugUAGUAUCAg -3' miRNA: 3'- -UCUGAaauuUGCGGAUcgAUCAUGGU- -5' |
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| 31283 | 3' | -46.5 | NC_006565.1 | + | 131110 | 0.67 | 0.99924 |
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Target: 5'- uGGAUcgUUAAGCaGCCUAGCaAGUAUa- -3' miRNA: 3'- -UCUGa-AAUUUG-CGGAUCGaUCAUGgu -5' |
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| 31283 | 3' | -46.5 | NC_006565.1 | + | 77419 | 0.68 | 0.997907 |
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Target: 5'- cGGcACUagAAGCgGCUUGGCUAGcUACCGa -3' miRNA: 3'- -UC-UGAaaUUUG-CGGAUCGAUC-AUGGU- -5' |
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| 31283 | 3' | -46.5 | NC_006565.1 | + | 64724 | 0.72 | 0.976413 |
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Target: 5'- cAGACUggucauacauACGCUUAGCUAGUAUUu -3' miRNA: 3'- -UCUGAaauu------UGCGGAUCGAUCAUGGu -5' |
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| 31283 | 3' | -46.5 | NC_006565.1 | + | 117842 | 0.75 | 0.909633 |
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Target: 5'- aAGACUUUcucagAGGCGUCUAGCUGGUuuaaaAUCAa -3' miRNA: 3'- -UCUGAAA-----UUUGCGGAUCGAUCA-----UGGU- -5' |
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| 31283 | 3' | -46.5 | NC_006565.1 | + | 2296 | 0.9 | 0.229895 |
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Target: 5'- uAGACUUUuaaaGCCUAGCUAGUACCAc -3' miRNA: 3'- -UCUGAAAuuugCGGAUCGAUCAUGGU- -5' |
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| 31283 | 3' | -46.5 | NC_006565.1 | + | 2250 | 1.1 | 0.017301 |
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Target: 5'- uAGACUUUAAACGCCUAGCUAGUACCAc -3' miRNA: 3'- -UCUGAAAUUUGCGGAUCGAUCAUGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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