Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31283 | 5' | -41.1 | NC_006565.1 | + | 8819 | 0.68 | 1 |
Target: 5'- -aCCAaacucUUUAGCUAGAUcUUUAAuAGCCu -3' miRNA: 3'- caGGU-----AAGUUGAUCUGaAAAUU-UCGG- -5' |
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31283 | 5' | -41.1 | NC_006565.1 | + | 98941 | 0.67 | 1 |
Target: 5'- -gCUAUUCAGaagauuagucuCUAGGCUUUUAcAGUCu -3' miRNA: 3'- caGGUAAGUU-----------GAUCUGAAAAUuUCGG- -5' |
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31283 | 5' | -41.1 | NC_006565.1 | + | 84190 | 0.66 | 1 |
Target: 5'- uUCUAuUUUAGCUAGuucACUgauaUUAGGGCCg -3' miRNA: 3'- cAGGU-AAGUUGAUC---UGAa---AAUUUCGG- -5' |
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31283 | 5' | -41.1 | NC_006565.1 | + | 38246 | 0.67 | 1 |
Target: 5'- uUCCAaaggcagcuacuagUUCAGCUaguucAGACga-UAAGGCCu -3' miRNA: 3'- cAGGU--------------AAGUUGA-----UCUGaaaAUUUCGG- -5' |
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31283 | 5' | -41.1 | NC_006565.1 | + | 65372 | 0.66 | 1 |
Target: 5'- cGUCagGUUCAGCUAGAUUuaaUUUAAucuuaaauucuGGCUa -3' miRNA: 3'- -CAGg-UAAGUUGAUCUGA---AAAUU-----------UCGG- -5' |
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31283 | 5' | -41.1 | NC_006565.1 | + | 109567 | 0.71 | 0.999993 |
Target: 5'- cUCCucgUCAGCUAucuguuGACUgg-GAGGCCu -3' miRNA: 3'- cAGGua-AGUUGAU------CUGAaaaUUUCGG- -5' |
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31283 | 5' | -41.1 | NC_006565.1 | + | 131245 | 0.72 | 0.999959 |
Target: 5'- aUCCAU--GACUggcGGGCgUUUAAAGCCa -3' miRNA: 3'- cAGGUAagUUGA---UCUGaAAAUUUCGG- -5' |
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31283 | 5' | -41.1 | NC_006565.1 | + | 2412 | 0.74 | 0.999614 |
Target: 5'- aUCCGUgguacUAGCUAGGCgUUUAAAGUCu -3' miRNA: 3'- cAGGUAa----GUUGAUCUGaAAAUUUCGG- -5' |
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31283 | 5' | -41.1 | NC_006565.1 | + | 2458 | 0.74 | 0.999377 |
Target: 5'- aUCCGUgguacUAGCUAGGCUUUaAAAGUCu -3' miRNA: 3'- cAGGUAa----GUUGAUCUGAAAaUUUCGG- -5' |
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31283 | 5' | -41.1 | NC_006565.1 | + | 2238 | 0.96 | 0.34314 |
Target: 5'- aGUCCAUUCAACUAGACUUUaAAcGCCu -3' miRNA: 3'- -CAGGUAAGUUGAUCUGAAAaUUuCGG- -5' |
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31283 | 5' | -41.1 | NC_006565.1 | + | 2284 | 1.16 | 0.033274 |
Target: 5'- aGUCCAUUCAACUAGACUUUUAAAGCCu -3' miRNA: 3'- -CAGGUAAGUUGAUCUGAAAAUUUCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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