Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 313 | 5' | -59.6 | AC_000010.1 | + | 4842 | 0.66 | 0.365963 |
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Target: 5'- cGGCCCC-ACUGGGUGUGgcaagucacAGCUGCUCa -3' miRNA: 3'- -UUGGGGcUGGCUCGCACa--------UCGGUGGG- -5' |
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| 313 | 5' | -59.6 | AC_000010.1 | + | 16144 | 0.66 | 0.348887 |
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Target: 5'- cACCaCCGGCCaGcGCGUGcccguGCgCACCCg -3' miRNA: 3'- uUGG-GGCUGG-CuCGCACau---CG-GUGGG- -5' |
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| 313 | 5' | -59.6 | AC_000010.1 | + | 6297 | 0.68 | 0.287092 |
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Target: 5'- cAGCCCCGAUuaugcaGGGUGauuagauccacacugGUGGCCACCUc -3' miRNA: 3'- -UUGGGGCUGg-----CUCGCa--------------CAUCGGUGGG- -5' |
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| 313 | 5' | -59.6 | AC_000010.1 | + | 26169 | 0.68 | 0.268042 |
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Target: 5'- -cCCCCGACCcgGAGCGgagaugGUAGCUgugguacugucugugGCCUu -3' miRNA: 3'- uuGGGGCUGG--CUCGCa-----CAUCGG---------------UGGG- -5' |
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| 313 | 5' | -59.6 | AC_000010.1 | + | 10259 | 0.68 | 0.254598 |
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Target: 5'- cGCCCCGGCUacaGAGCGaUGgccccuauaccgccAGCCGCCa -3' miRNA: 3'- uUGGGGCUGG---CUCGC-ACa-------------UCGGUGGg -5' |
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| 313 | 5' | -59.6 | AC_000010.1 | + | 24950 | 0.7 | 0.198706 |
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Target: 5'- cGACUCgGACaGAGCGUGUugcacaCCACCCu -3' miRNA: 3'- -UUGGGgCUGgCUCGCACAuc----GGUGGG- -5' |
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| 313 | 5' | -59.6 | AC_000010.1 | + | 6393 | 0.74 | 0.096447 |
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Target: 5'- gAGCCCCu-CCGAgGCGagGUGGCCACCa -3' miRNA: 3'- -UUGGGGcuGGCU-CGCa-CAUCGGUGGg -5' |
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| 313 | 5' | -59.6 | AC_000010.1 | + | 9677 | 1.08 | 0.000242 |
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Target: 5'- gAACCCCGACCGAGCGUGUAGCCACCCc -3' miRNA: 3'- -UUGGGGCUGGCUCGCACAUCGGUGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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