Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 31300 | 5' | -61.3 | NC_006623.1 | + | 129705 | 0.72 | 0.309208 |
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Target: 5'- gGGAGGAACCauccaagagaacCUGGCGGUGCUCGcGAa -3' miRNA: 3'- gCCUCCUUGG------------GACCGCCGUGGGU-CUa -5' |
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| 31300 | 5' | -61.3 | NC_006623.1 | + | 139941 | 0.68 | 0.517458 |
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Target: 5'- gCGGGGGAuaGCaaaUGGCGGCgGCgCCGGAg -3' miRNA: 3'- -GCCUCCU--UGgg-ACCGCCG-UG-GGUCUa -5' |
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| 31300 | 5' | -61.3 | NC_006623.1 | + | 128965 | 0.68 | 0.536663 |
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Target: 5'- uGGcaauGGAuCCCUGGUGcuGUACCCAGGg -3' miRNA: 3'- gCCu---CCUuGGGACCGC--CGUGGGUCUa -5' |
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| 31300 | 5' | -61.3 | NC_006623.1 | + | 57457 | 0.68 | 0.546359 |
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Target: 5'- uGGA--AGCCCUGGCGaGUuCCCGGAc -3' miRNA: 3'- gCCUccUUGGGACCGC-CGuGGGUCUa -5' |
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| 31300 | 5' | -61.3 | NC_006623.1 | + | 137552 | 0.67 | 0.595553 |
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Target: 5'- cCGGAGGGagAUUCUGGCGGgCGuCgCAGAg -3' miRNA: 3'- -GCCUCCU--UGGGACCGCC-GU-GgGUCUa -5' |
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| 31300 | 5' | -61.3 | NC_006623.1 | + | 124060 | 0.67 | 0.595553 |
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Target: 5'- cCGGAGGGagAUUCUGGCGGgCGuCgCAGAg -3' miRNA: 3'- -GCCUCCU--UGGGACCGCC-GU-GgGUCUa -5' |
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| 31300 | 5' | -61.3 | NC_006623.1 | + | 121671 | 0.68 | 0.517458 |
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Target: 5'- gCGGGGGAuaGCaaaUGGCGGCgGCgCCGGAg -3' miRNA: 3'- -GCCUCCU--UGgg-ACCGCCG-UG-GGUCUa -5' |
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| 31300 | 5' | -61.3 | NC_006623.1 | + | 1391 | 1.06 | 0.001424 |
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Target: 5'- gCGGAGGAACCCUGGCGGCACCCAGAUa -3' miRNA: 3'- -GCCUCCUUGGGACCGCCGUGGGUCUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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