Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31301 | 5' | -55.2 | NC_006623.1 | + | 131224 | 0.66 | 0.917026 |
Target: 5'- --cGCUCGcGCUCCga--GCGCGGCg- -3' miRNA: 3'- acaUGAGC-CGAGGauagCGUGUCGga -5' |
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31301 | 5' | -55.2 | NC_006623.1 | + | 8789 | 0.66 | 0.911065 |
Target: 5'- --gGgUUGGCUUCUGUUGCAgAGaCCUg -3' miRNA: 3'- acaUgAGCCGAGGAUAGCGUgUC-GGA- -5' |
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31301 | 5' | -55.2 | NC_006623.1 | + | 13881 | 0.67 | 0.891043 |
Target: 5'- cGUACUCgcaaauauuaaauGGCUCCUGccCGgACAGCa- -3' miRNA: 3'- aCAUGAG-------------CCGAGGAUa-GCgUGUCGga -5' |
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31301 | 5' | -55.2 | NC_006623.1 | + | 116435 | 0.67 | 0.87325 |
Target: 5'- cGUACggugacacagauaaUGGCUCCUguucAUCGCACGGUg- -3' miRNA: 3'- aCAUGa-------------GCCGAGGA----UAGCGUGUCGga -5' |
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31301 | 5' | -55.2 | NC_006623.1 | + | 145177 | 0.67 | 0.87325 |
Target: 5'- cGUACggugacacagauaaUGGCUCCUguucAUCGCACGGUg- -3' miRNA: 3'- aCAUGa-------------GCCGAGGA----UAGCGUGUCGga -5' |
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31301 | 5' | -55.2 | NC_006623.1 | + | 14211 | 0.67 | 0.862673 |
Target: 5'- cGUAUccacccCGGCUCCUGuccugucuUCGCACGGUg- -3' miRNA: 3'- aCAUGa-----GCCGAGGAU--------AGCGUGUCGga -5' |
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31301 | 5' | -55.2 | NC_006623.1 | + | 63770 | 0.68 | 0.846837 |
Target: 5'- aGUGucgaGGCUCCUGUCGCAUGcGUCa -3' miRNA: 3'- aCAUgag-CCGAGGAUAGCGUGU-CGGa -5' |
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31301 | 5' | -55.2 | NC_006623.1 | + | 144663 | 0.68 | 0.830196 |
Target: 5'- cGUGCUUGGCUgCUccgacgaggguGaCGUGCGGCCUu -3' miRNA: 3'- aCAUGAGCCGAgGA-----------UaGCGUGUCGGA- -5' |
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31301 | 5' | -55.2 | NC_006623.1 | + | 144859 | 0.68 | 0.830196 |
Target: 5'- gGUACgaccuaccuccCGGCacUCCcgAUCGCGCGGCCc -3' miRNA: 3'- aCAUGa----------GCCG--AGGa-UAGCGUGUCGGa -5' |
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31301 | 5' | -55.2 | NC_006623.1 | + | 116949 | 0.68 | 0.830196 |
Target: 5'- cGUGCUUGGCUgCUccgacgaggguGaCGUGCGGCCUu -3' miRNA: 3'- aCAUGAGCCGAgGA-----------UaGCGUGUCGGA- -5' |
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31301 | 5' | -55.2 | NC_006623.1 | + | 137201 | 0.69 | 0.803861 |
Target: 5'- --cGCUCGGCaUCCUcUCGCGC-GUCg -3' miRNA: 3'- acaUGAGCCG-AGGAuAGCGUGuCGGa -5' |
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31301 | 5' | -55.2 | NC_006623.1 | + | 116753 | 0.69 | 0.803861 |
Target: 5'- cGUACgaccuaccuccCGGCacUCCcgAUCGCGCGGCCc -3' miRNA: 3'- aCAUGa----------GCCG--AGGa-UAGCGUGUCGGa -5' |
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31301 | 5' | -55.2 | NC_006623.1 | + | 1601 | 1.07 | 0.003834 |
Target: 5'- gUGUACUCGGCUCCUAUCGCACAGCCUu -3' miRNA: 3'- -ACAUGAGCCGAGGAUAGCGUGUCGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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