Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31302 | 3' | -53.3 | NC_006623.1 | + | 1805 | 1.07 | 0.006237 |
Target: 5'- uCUCCGAACCCACCCAGAGACAAGAUAg -3' miRNA: 3'- -GAGGCUUGGGUGGGUCUCUGUUCUAU- -5' |
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31302 | 3' | -53.3 | NC_006623.1 | + | 117856 | 0.67 | 0.945836 |
Target: 5'- aUCCcaucCCCACCCAGuagaGGACAAGuAUAu -3' miRNA: 3'- gAGGcuu-GGGUGGGUC----UCUGUUC-UAU- -5' |
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31302 | 3' | -53.3 | NC_006623.1 | + | 124070 | 0.69 | 0.888191 |
Target: 5'- --aCGGACCUGCCCGGAGG-GAGAUu -3' miRNA: 3'- gagGCUUGGGUGGGUCUCUgUUCUAu -5' |
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31302 | 3' | -53.3 | NC_006623.1 | + | 128151 | 0.66 | 0.950226 |
Target: 5'- uCUCCGAGCUgGCCCuGAacaaaaauGGCGGGGc- -3' miRNA: 3'- -GAGGCUUGGgUGGGuCU--------CUGUUCUau -5' |
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31302 | 3' | -53.3 | NC_006623.1 | + | 131400 | 0.66 | 0.954373 |
Target: 5'- cCUCCGGcGCCgCGCUCGGAGcGCGAGcgGu -3' miRNA: 3'- -GAGGCU-UGG-GUGGGUCUC-UGUUCuaU- -5' |
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31302 | 3' | -53.3 | NC_006623.1 | + | 137542 | 0.69 | 0.888191 |
Target: 5'- --aCGGACCUGCCCGGAGG-GAGAUu -3' miRNA: 3'- gagGCUUGGGUGGGUCUCUgUUCUAu -5' |
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31302 | 3' | -53.3 | NC_006623.1 | + | 143755 | 0.67 | 0.945836 |
Target: 5'- aUCCcaucCCCACCCAGuagaGGACAAGuAUAu -3' miRNA: 3'- gAGGcuu-GGGUGGGUC----UCUGUUC-UAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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