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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
|
P value |
| Predicted miRNA align pattern | |||||||
| 31303 | 3' | -48.7 | NC_006623.1 | + | 117351 | 0.67 | 0.996202 |
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Target: 5'- cCAUCac-GCAUGGCGuGCGCUaCGUGc -3' miRNA: 3'- -GUAGauaCGUAUUGCuCGUGAgGCAC- -5' |
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| 31303 | 3' | -48.7 | NC_006623.1 | + | 133092 | 0.67 | 0.997294 |
|
Target: 5'- aCAUCUGUGCAgauugAACGGcGCAUUCa--- -3' miRNA: 3'- -GUAGAUACGUa----UUGCU-CGUGAGgcac -5' |
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| 31303 | 3' | -48.7 | NC_006623.1 | + | 144261 | 0.67 | 0.996202 |
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Target: 5'- cCAUCac-GCAUGGCGuGCGCUaCGUGc -3' miRNA: 3'- -GUAGauaCGUAUUGCuCGUGAgGCAC- -5' |
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| 31303 | 3' | -48.7 | NC_006623.1 | + | 93940 | 0.68 | 0.993908 |
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Target: 5'- -uUCacgGuCAUGACGAGUGCUCCGg- -3' miRNA: 3'- guAGauaC-GUAUUGCUCGUGAGGCac -5' |
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| 31303 | 3' | -48.7 | NC_006623.1 | + | 22295 | 0.72 | 0.940762 |
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Target: 5'- -cUCUAUGCcaAGCG-GUugUCCGUGg -3' miRNA: 3'- guAGAUACGuaUUGCuCGugAGGCAC- -5' |
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| 31303 | 3' | -48.7 | NC_006623.1 | + | 1714 | 0.77 | 0.751297 |
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Target: 5'- uCGUgUAUGUAUAGCGAGCAaugaCCGUGu -3' miRNA: 3'- -GUAgAUACGUAUUGCUCGUga--GGCAC- -5' |
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| 31303 | 3' | -48.7 | NC_006623.1 | + | 1929 | 1.1 | 0.012284 |
|
Target: 5'- gCAUCUAUGCAUAACGAGCACUCCGUGa -3' miRNA: 3'- -GUAGAUACGUAUUGCUCGUGAGGCAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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