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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31304 | 5' | -48.4 | NC_006623.1 | + | 131828 | 0.66 | 0.99871 |
Target: 5'- cCGAACUGGUaGGGUUUugcgaggcCAGGGGuGUGc -3' miRNA: 3'- -GUUUGACCA-CUCAAAua------GUCUCC-CGC- -5' |
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31304 | 5' | -48.4 | NC_006623.1 | + | 143239 | 0.67 | 0.996202 |
Target: 5'- aGAAgUGGUGAGgaaGUCAGAGaGaGUGg -3' miRNA: 3'- gUUUgACCACUCaaaUAGUCUC-C-CGC- -5' |
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31304 | 5' | -48.4 | NC_006623.1 | + | 118373 | 0.67 | 0.996202 |
Target: 5'- aGAAgUGGUGAGgaaGUCAGAGaGaGUGg -3' miRNA: 3'- gUUUgACCACUCaaaUAGUCUC-C-CGC- -5' |
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31304 | 5' | -48.4 | NC_006623.1 | + | 137240 | 0.68 | 0.994771 |
Target: 5'- aCGAGCUGGcucg--UAUgCGGAGGGCGu -3' miRNA: 3'- -GUUUGACCacucaaAUA-GUCUCCCGC- -5' |
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31304 | 5' | -48.4 | NC_006623.1 | + | 2088 | 1.1 | 0.012284 |
Target: 5'- gCAAACUGGUGAGUUUAUCAGAGGGCGa -3' miRNA: 3'- -GUUUGACCACUCAAAUAGUCUCCCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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