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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31309 | 3' | -51.4 | NC_006623.1 | + | 123456 | 0.68 | 0.956834 |
Target: 5'- cGCgagUCCggcgGcCGGCGCGCUGcUCugGUUa -3' miRNA: 3'- -UGa--AGGa---CaGUUGCGUGAC-AGugCAG- -5' |
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31309 | 3' | -51.4 | NC_006623.1 | + | 65948 | 0.67 | 0.973514 |
Target: 5'- aACUUCCUuUC--CGCGCUGUUACaUCg -3' miRNA: 3'- -UGAAGGAcAGuuGCGUGACAGUGcAG- -5' |
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31309 | 3' | -51.4 | NC_006623.1 | + | 138156 | 0.68 | 0.956834 |
Target: 5'- cGCgagUCCggcgGcCGGCGCGCUGcUCugGUUa -3' miRNA: 3'- -UGa--AGGa---CaGUUGCGUGAC-AGugCAG- -5' |
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31309 | 3' | -51.4 | NC_006623.1 | + | 86425 | 0.71 | 0.877193 |
Target: 5'- uACUUCCauUG-CGACGCGCUGgugaaugaaUCAUGUCc -3' miRNA: 3'- -UGAAGG--ACaGUUGCGUGAC---------AGUGCAG- -5' |
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31309 | 3' | -51.4 | NC_006623.1 | + | 3026 | 1.1 | 0.006654 |
Target: 5'- cACUUCCUGUCAACGCACUGUCACGUCu -3' miRNA: 3'- -UGAAGGACAGUUGCGUGACAGUGCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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