Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31309 | 5' | -48.7 | NC_006623.1 | + | 126340 | 0.67 | 0.993958 |
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Target: 5'- uGAGCGaCGGGACcGUAUguccGGCGCGAu -3' miRNA: 3'- uUUUGC-GCUUUGaCGUAa---CCGUGCUu -5' |
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| 31309 | 5' | -48.7 | NC_006623.1 | + | 116684 | 0.67 | 0.991854 |
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Target: 5'- -uGACGUGcucGCUGCcg-GGCGCGGAa -3' miRNA: 3'- uuUUGCGCuu-UGACGuaaCCGUGCUU- -5' |
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| 31309 | 5' | -48.7 | NC_006623.1 | + | 128795 | 0.67 | 0.991854 |
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Target: 5'- ----aGCGAAACggGCAgUGGaCACGAGg -3' miRNA: 3'- uuuugCGCUUUGa-CGUaACC-GUGCUU- -5' |
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| 31309 | 5' | -48.7 | NC_006623.1 | + | 144928 | 0.67 | 0.991854 |
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Target: 5'- -uGACGUGcucGCUGCcg-GGCGCGGAa -3' miRNA: 3'- uuUUGCGCuu-UGACGuaaCCGUGCUU- -5' |
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| 31309 | 5' | -48.7 | NC_006623.1 | + | 111840 | 0.69 | 0.978196 |
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Target: 5'- --cACGCGAAagugaccauugauguACUGCAUUGcGuCGCGGAa -3' miRNA: 3'- uuuUGCGCUU---------------UGACGUAAC-C-GUGCUU- -5' |
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| 31309 | 5' | -48.7 | NC_006623.1 | + | 24671 | 0.69 | 0.977173 |
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Target: 5'- --uGCGCGAaaaAugUGCg--GGCGCGAGa -3' miRNA: 3'- uuuUGCGCU---UugACGuaaCCGUGCUU- -5' |
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| 31309 | 5' | -48.7 | NC_006623.1 | + | 2991 | 0.9 | 0.160388 |
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Target: 5'- gAAAACGCGAAA-UGCAUUGGCACGAAu -3' miRNA: 3'- -UUUUGCGCUUUgACGUAACCGUGCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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