Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31310 | 3' | -57.8 | NC_006623.1 | + | 4532 | 1.09 | 0.001901 |
Target: 5'- aAGGACGCAAGGCCUGUCCUGUGUCCGa -3' miRNA: 3'- -UCCUGCGUUCCGGACAGGACACAGGC- -5' |
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31310 | 3' | -57.8 | NC_006623.1 | + | 15424 | 0.68 | 0.710865 |
Target: 5'- uGGcUGCAAaaGCCUGUCC-GUGUCCa -3' miRNA: 3'- uCCuGCGUUc-CGGACAGGaCACAGGc -5' |
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31310 | 3' | -57.8 | NC_006623.1 | + | 111988 | 0.73 | 0.436351 |
Target: 5'- aAGGAUGCAGGGUgUGUgcaCUGgugGUCCGc -3' miRNA: 3'- -UCCUGCGUUCCGgACAg--GACa--CAGGC- -5' |
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31310 | 3' | -57.8 | NC_006623.1 | + | 122690 | 0.71 | 0.520409 |
Target: 5'- cGGACGCGAGGUCgg-CCggcagGUGUCgGg -3' miRNA: 3'- uCCUGCGUUCCGGacaGGa----CACAGgC- -5' |
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31310 | 3' | -57.8 | NC_006623.1 | + | 138922 | 0.71 | 0.520409 |
Target: 5'- cGGACGCGAGGUCgg-CCggcagGUGUCgGg -3' miRNA: 3'- uCCUGCGUUCCGGacaGGa----CACAGgC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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