Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 31310 | 5' | -52.2 | NC_006623.1 | + | 1236 | 0.66 | 0.974995 |
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Target: 5'- aGACCGGcCUGGcACGGACCC-----CCa -3' miRNA: 3'- -CUGGCU-GACC-UGUCUGGGuuuaaGGa -5' |
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| 31310 | 5' | -52.2 | NC_006623.1 | + | 1844 | 0.68 | 0.951086 |
|
Target: 5'- -cUCGACUGGGCAGagacuagauGCCCGAAaaUCCc -3' miRNA: 3'- cuGGCUGACCUGUC---------UGGGUUUa-AGGa -5' |
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| 31310 | 5' | -52.2 | NC_006623.1 | + | 4498 | 1.08 | 0.006876 |
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Target: 5'- gGACCGACUGGACAGACCCAAAUUCCUg -3' miRNA: 3'- -CUGGCUGACCUGUCUGGGUUUAAGGA- -5' |
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| 31310 | 5' | -52.2 | NC_006623.1 | + | 21002 | 0.69 | 0.926796 |
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Target: 5'- uGACCaGCgcGGGgGGACCUGGAUUCCg -3' miRNA: 3'- -CUGGcUGa-CCUgUCUGGGUUUAAGGa -5' |
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| 31310 | 5' | -52.2 | NC_006623.1 | + | 28580 | 0.66 | 0.982049 |
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Target: 5'- cACCGACUGGAaauaucuGGgCCGAuUUCCc -3' miRNA: 3'- cUGGCUGACCUgu-----CUgGGUUuAAGGa -5' |
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| 31310 | 5' | -52.2 | NC_006623.1 | + | 38405 | 0.71 | 0.851485 |
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Target: 5'- aGACCGGCUcuuuacgaAGACCCAAAUUUCUg -3' miRNA: 3'- -CUGGCUGAccug----UCUGGGUUUAAGGA- -5' |
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| 31310 | 5' | -52.2 | NC_006623.1 | + | 64642 | 0.69 | 0.92118 |
|
Target: 5'- -uCUGAUUGGGgAGACCCAuAUUCg- -3' miRNA: 3'- cuGGCUGACCUgUCUGGGUuUAAGga -5' |
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| 31310 | 5' | -52.2 | NC_006623.1 | + | 122102 | 0.68 | 0.942124 |
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Target: 5'- cGGCCGACUcGG-UAGACCCGugcAUUCg- -3' miRNA: 3'- -CUGGCUGA-CCuGUCUGGGUu--UAAGga -5' |
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| 31310 | 5' | -52.2 | NC_006623.1 | + | 128602 | 0.72 | 0.789898 |
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Target: 5'- cGCCGguACUGGACGGGCUCGAGUcgagCCc -3' miRNA: 3'- cUGGC--UGACCUGUCUGGGUUUAa---GGa -5' |
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| 31310 | 5' | -52.2 | NC_006623.1 | + | 139510 | 0.68 | 0.942124 |
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Target: 5'- cGGCCGACUcGG-UAGACCCGugcAUUCg- -3' miRNA: 3'- -CUGGCUGA-CCuGUCUGGGUu--UAAGga -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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