miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
31311 3' -54.3 NC_006623.1 + 8163 1.07 0.004436
Target:  5'- uUUGCUGCACACCAAUUGCGUCGCCGAg -3'
miRNA:   3'- -AACGACGUGUGGUUAACGCAGCGGCU- -5'
31311 3' -54.3 NC_006623.1 + 31122 0.74 0.55164
Target:  5'- -aGCcGUACACgucuaAAUUGCGUUGCCGAg -3'
miRNA:   3'- aaCGaCGUGUGg----UUAACGCAGCGGCU- -5'
31311 3' -54.3 NC_006623.1 + 111855 0.69 0.806796
Target:  5'- aUUGaUGUACugC-AUUGCGUCGCgGAa -3'
miRNA:   3'- -AACgACGUGugGuUAACGCAGCGgCU- -5'
31311 3' -54.3 NC_006623.1 + 131224 0.68 0.841821
Target:  5'- -cGCUcGCGCuCCGAgcGCGgCGCCGGa -3'
miRNA:   3'- aaCGA-CGUGuGGUUaaCGCaGCGGCU- -5'
31311 3' -54.3 NC_006623.1 + 7950 0.68 0.85815
Target:  5'- -cGCUcacGCAgGCCGGU--CGUCGCCGGu -3'
miRNA:   3'- aaCGA---CGUgUGGUUAacGCAGCGGCU- -5'
31311 3' -54.3 NC_006623.1 + 121839 0.67 0.895062
Target:  5'- -aGCggacGCGCGCCccuUUGCGacCGCCGGa -3'
miRNA:   3'- aaCGa---CGUGUGGuu-AACGCa-GCGGCU- -5'
31311 3' -54.3 NC_006623.1 + 139772 0.67 0.895062
Target:  5'- -aGCggacGCGCGCCccuUUGCGacCGCCGGa -3'
miRNA:   3'- aaCGa---CGUGUGGuu-AACGCa-GCGGCU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.