Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31313 | 5' | -52.3 | NC_006623.1 | + | 7751 | 0.66 | 0.9871 |
Target: 5'- cGCCGgu--CGCUGuAGauacauugucauaGUCAGAGUCCc -3' miRNA: 3'- cCGGCaaguGCGAC-UC-------------CAGUCUUAGG- -5' |
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31313 | 5' | -52.3 | NC_006623.1 | + | 7936 | 0.66 | 0.985609 |
Target: 5'- aGGCucuCGUggaUCuuGCUgGGGGUCAGGuUCCu -3' miRNA: 3'- -CCG---GCA---AGugCGA-CUCCAGUCUuAGG- -5' |
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31313 | 5' | -52.3 | NC_006623.1 | + | 130045 | 0.66 | 0.983802 |
Target: 5'- cGuuGUcCGCGuCUGAGG-CGGAcGUCCc -3' miRNA: 3'- cCggCAaGUGC-GACUCCaGUCU-UAGG- -5' |
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31313 | 5' | -52.3 | NC_006623.1 | + | 123467 | 0.67 | 0.977345 |
Target: 5'- cGGCCGg-CGCGCUGcucuGGUUAu--UCCa -3' miRNA: 3'- -CCGGCaaGUGCGACu---CCAGUcuuAGG- -5' |
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31313 | 5' | -52.3 | NC_006623.1 | + | 138145 | 0.67 | 0.977345 |
Target: 5'- cGGCCGg-CGCGCUGcucuGGUUAu--UCCa -3' miRNA: 3'- -CCGGCaaGUGCGACu---CCAGUcuuAGG- -5' |
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31313 | 5' | -52.3 | NC_006623.1 | + | 107701 | 0.67 | 0.974558 |
Target: 5'- cGG-CGUUUuuuagcaGCGUUGGGGUCGGuAUCUc -3' miRNA: 3'- -CCgGCAAG-------UGCGACUCCAGUCuUAGG- -5' |
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31313 | 5' | -52.3 | NC_006623.1 | + | 184 | 0.67 | 0.966026 |
Target: 5'- cGCCGUUCGgucgcggcCGCgaGGGGUUucGAAUCC -3' miRNA: 3'- cCGGCAAGU--------GCGa-CUCCAGu-CUUAGG -5' |
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31313 | 5' | -52.3 | NC_006623.1 | + | 71528 | 0.7 | 0.883617 |
Target: 5'- -aCUGgaaAUGCUGGGGUCAG-AUCCg -3' miRNA: 3'- ccGGCaagUGCGACUCCAGUCuUAGG- -5' |
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31313 | 5' | -52.3 | NC_006623.1 | + | 69577 | 0.71 | 0.877151 |
Target: 5'- uGGuCUGUgCGCGCUGcucugggaagugcuuGGUCAGAGUCUc -3' miRNA: 3'- -CC-GGCAaGUGCGACu--------------CCAGUCUUAGG- -5' |
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31313 | 5' | -52.3 | NC_006623.1 | + | 130609 | 0.73 | 0.745342 |
Target: 5'- aGGCCGUaaACGC-GAGGacgcugcCGGAGUCCg -3' miRNA: 3'- -CCGGCAagUGCGaCUCCa------GUCUUAGG- -5' |
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31313 | 5' | -52.3 | NC_006623.1 | + | 12340 | 1.14 | 0.00359 |
Target: 5'- uGGCCGUUCACGCUGAGGUCAGAAUCCc -3' miRNA: 3'- -CCGGCAAGUGCGACUCCAGUCUUAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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