Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31314 | 3' | -54.8 | NC_006623.1 | + | 15133 | 0.66 | 0.948663 |
Target: 5'- ----aCUgCUCGUCC--CCGGAGAGCc -3' miRNA: 3'- gauaaGGgGGGUAGGaaGGCCUCUCG- -5' |
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31314 | 3' | -54.8 | NC_006623.1 | + | 124789 | 0.66 | 0.924346 |
Target: 5'- -aAUUCUUUCCAUCCagaaUCCG-AGAGCu -3' miRNA: 3'- gaUAAGGGGGGUAGGa---AGGCcUCUCG- -5' |
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31314 | 3' | -54.8 | NC_006623.1 | + | 133571 | 0.66 | 0.924346 |
Target: 5'- gUcgUCCUCCgAgUCUUCUGGAGGGg -3' miRNA: 3'- gAuaAGGGGGgUaGGAAGGCCUCUCg -5' |
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31314 | 3' | -54.8 | NC_006623.1 | + | 28048 | 0.67 | 0.917036 |
Target: 5'- ----aCgCCCCAaucgCCUUCCcagaagaguuucucGGAGAGCa -3' miRNA: 3'- gauaaGgGGGGUa---GGAAGG--------------CCUCUCG- -5' |
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31314 | 3' | -54.8 | NC_006623.1 | + | 125390 | 0.68 | 0.887242 |
Target: 5'- -aGUUCUgCCCGUUCUcguuagCCGGGGGGa -3' miRNA: 3'- gaUAAGGgGGGUAGGAa-----GGCCUCUCg -5' |
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31314 | 3' | -54.8 | NC_006623.1 | + | 130594 | 0.68 | 0.865574 |
Target: 5'- aUGUUCCCagggacgacUCUAUCCgUCCGG-GGGCc -3' miRNA: 3'- gAUAAGGG---------GGGUAGGaAGGCCuCUCG- -5' |
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31314 | 3' | -54.8 | NC_006623.1 | + | 145360 | 0.69 | 0.83374 |
Target: 5'- ----gCCCUaCCAUCCUguucgcaCCGGAGGGUu -3' miRNA: 3'- gauaaGGGG-GGUAGGAa------GGCCUCUCG- -5' |
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31314 | 3' | -54.8 | NC_006623.1 | + | 73625 | 0.69 | 0.83374 |
Target: 5'- aUcgUCUCCUCGcgUCUaCCGGGGAGCu -3' miRNA: 3'- gAuaAGGGGGGUa-GGAaGGCCUCUCG- -5' |
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31314 | 3' | -54.8 | NC_006623.1 | + | 116252 | 0.69 | 0.83374 |
Target: 5'- ----gCCCUaCCAUCCUguucgcaCCGGAGGGUu -3' miRNA: 3'- gauaaGGGG-GGUAGGAa------GGCCUCUCG- -5' |
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31314 | 3' | -54.8 | NC_006623.1 | + | 86108 | 0.69 | 0.8253 |
Target: 5'- -gAUUCaaaaaCCCCAUCaccgUgUGGAGAGCa -3' miRNA: 3'- gaUAAGg----GGGGUAGga--AgGCCUCUCG- -5' |
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31314 | 3' | -54.8 | NC_006623.1 | + | 88239 | 0.7 | 0.789843 |
Target: 5'- ---cUCUUCUCAUCUUUCCuGGAGGGUu -3' miRNA: 3'- gauaAGGGGGGUAGGAAGG-CCUCUCG- -5' |
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31314 | 3' | -54.8 | NC_006623.1 | + | 75839 | 0.7 | 0.779665 |
Target: 5'- ---aUCCCCCCuugcuagAUCCa--UGGAGAGCu -3' miRNA: 3'- gauaAGGGGGG-------UAGGaagGCCUCUCG- -5' |
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31314 | 3' | -54.8 | NC_006623.1 | + | 112175 | 0.75 | 0.509561 |
Target: 5'- ----cCCCCCCAaagUUCUUCCacccguGGAGAGCa -3' miRNA: 3'- gauaaGGGGGGU---AGGAAGG------CCUCUCG- -5' |
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31314 | 3' | -54.8 | NC_006623.1 | + | 24409 | 1.12 | 0.002494 |
Target: 5'- cCUAUUCCCCCCAUCCUUCCGGAGAGCu -3' miRNA: 3'- -GAUAAGGGGGGUAGGAAGGCCUCUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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