Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31314 | 5' | -53.7 | NC_006623.1 | + | 69598 | 0.66 | 0.940586 |
Target: 5'- cAUGU-UCCCGA--AUACGGGCuGGAACc -3' miRNA: 3'- -UACGcGGGGCUuuUAUGUCCG-CCUUG- -5' |
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31314 | 5' | -53.7 | NC_006623.1 | + | 112267 | 0.67 | 0.930442 |
Target: 5'- ---aGCCCCGGAAAagguuCAGGUGGGc- -3' miRNA: 3'- uacgCGGGGCUUUUau---GUCCGCCUug -5' |
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31314 | 5' | -53.7 | NC_006623.1 | + | 128930 | 0.67 | 0.929908 |
Target: 5'- cUGCGCUCCG-------GGGUGGAACu -3' miRNA: 3'- uACGCGGGGCuuuuaugUCCGCCUUG- -5' |
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31314 | 5' | -53.7 | NC_006623.1 | + | 125092 | 0.67 | 0.917519 |
Target: 5'- uUGCGCCCCu---AUGCAGaaauuuugccgaccGCGGAAg -3' miRNA: 3'- uACGCGGGGcuuuUAUGUC--------------CGCCUUg -5' |
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31314 | 5' | -53.7 | NC_006623.1 | + | 15769 | 0.67 | 0.913319 |
Target: 5'- aGUGUGCCCCGuAGcgACAGagaguauCGGAAUa -3' miRNA: 3'- -UACGCGGGGCuUUuaUGUCc------GCCUUG- -5' |
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31314 | 5' | -53.7 | NC_006623.1 | + | 110794 | 0.67 | 0.907103 |
Target: 5'- -aGCGCUCCGGAGcUACAGGUcGcACc -3' miRNA: 3'- uaCGCGGGGCUUUuAUGUCCGcCuUG- -5' |
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31314 | 5' | -53.7 | NC_006623.1 | + | 128472 | 0.67 | 0.900638 |
Target: 5'- cUGCGCCC----GGUACGGGCGcgcGAACu -3' miRNA: 3'- uACGCGGGgcuuUUAUGUCCGC---CUUG- -5' |
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31314 | 5' | -53.7 | NC_006623.1 | + | 53650 | 0.68 | 0.886967 |
Target: 5'- gGUGCGCUUCGuGAAUGCuGGauuaagccuugcCGGAACg -3' miRNA: 3'- -UACGCGGGGCuUUUAUGuCC------------GCCUUG- -5' |
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31314 | 5' | -53.7 | NC_006623.1 | + | 102085 | 0.68 | 0.886967 |
Target: 5'- -cGCGCCUaugaUGGAAAUACuGG-GGAGCu -3' miRNA: 3'- uaCGCGGG----GCUUUUAUGuCCgCCUUG- -5' |
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31314 | 5' | -53.7 | NC_006623.1 | + | 134463 | 0.68 | 0.872337 |
Target: 5'- -aGCGCCuCCGAAc-UugAaGCGGAACa -3' miRNA: 3'- uaCGCGG-GGCUUuuAugUcCGCCUUG- -5' |
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31314 | 5' | -53.7 | NC_006623.1 | + | 116940 | 0.68 | 0.871581 |
Target: 5'- -gGCGCCCuCGAGAGUGCuugauacagauccGGGCGacGCu -3' miRNA: 3'- uaCGCGGG-GCUUUUAUG-------------UCCGCcuUG- -5' |
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31314 | 5' | -53.7 | NC_006623.1 | + | 144672 | 0.68 | 0.871581 |
Target: 5'- -gGCGCCCuCGAGAGUGCuugauacagauccGGGCGacGCu -3' miRNA: 3'- uaCGCGGG-GCUUUUAUG-------------UCCGCcuUG- -5' |
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31314 | 5' | -53.7 | NC_006623.1 | + | 36988 | 0.69 | 0.823157 |
Target: 5'- -cGCGCCCCGuu-AUGCAGaGUGcAGCu -3' miRNA: 3'- uaCGCGGGGCuuuUAUGUC-CGCcUUG- -5' |
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31314 | 5' | -53.7 | NC_006623.1 | + | 24375 | 1.01 | 0.013412 |
Target: 5'- aAUGCGCCCCGAAAAUACAGGCGGAc- -3' miRNA: 3'- -UACGCGGGGCUUUUAUGUCCGCCUug -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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