Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31315 | 3' | -60.7 | NC_006623.1 | + | 475 | 0.68 | 0.616209 |
Target: 5'- uGCCgCGcuuuuaAUGGCGCUcgcgcuGGGugCUUGCCCg -3' miRNA: 3'- -UGG-GCa-----UACCGCGG------CCUugGGACGGG- -5' |
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31315 | 3' | -60.7 | NC_006623.1 | + | 9008 | 0.68 | 0.567026 |
Target: 5'- uACCCGUGcuccucGGUGUuguccuuccaCGGAAgUCUGCCCa -3' miRNA: 3'- -UGGGCAUa-----CCGCG----------GCCUUgGGACGGG- -5' |
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31315 | 3' | -60.7 | NC_006623.1 | + | 25291 | 1.1 | 0.000875 |
Target: 5'- gACCCGUAUGGCGCCGGAACCCUGCCCu -3' miRNA: 3'- -UGGGCAUACCGCGGCCUUGGGACGGG- -5' |
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31315 | 3' | -60.7 | NC_006623.1 | + | 115233 | 0.71 | 0.411238 |
Target: 5'- aGCCCcauGUG-GGCGCCGGGGCgaUGCCg -3' miRNA: 3'- -UGGG---CAUaCCGCGGCCUUGggACGGg -5' |
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31315 | 3' | -60.7 | NC_006623.1 | + | 116590 | 0.66 | 0.685319 |
Target: 5'- -aCCGUAcGG-GCCcGAACCCgaccGCCCu -3' miRNA: 3'- ugGGCAUaCCgCGGcCUUGGGa---CGGG- -5' |
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31315 | 3' | -60.7 | NC_006623.1 | + | 118102 | 0.66 | 0.695089 |
Target: 5'- aGCCC------CGaCCGGGACUCUGCCCa -3' miRNA: 3'- -UGGGcauaccGC-GGCCUUGGGACGGG- -5' |
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31315 | 3' | -60.7 | NC_006623.1 | + | 126347 | 0.71 | 0.428236 |
Target: 5'- uUCCGUAUGaGCGaCGGGACCguaUGUCCg -3' miRNA: 3'- uGGGCAUAC-CGCgGCCUUGGg--ACGGG- -5' |
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31315 | 3' | -60.7 | NC_006623.1 | + | 137356 | 0.67 | 0.655792 |
Target: 5'- -gCCGagcgAUGGCGCCucGACCgUGCCUu -3' miRNA: 3'- ugGGCa---UACCGCGGccUUGGgACGGG- -5' |
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31315 | 3' | -60.7 | NC_006623.1 | + | 143510 | 0.66 | 0.695089 |
Target: 5'- aGCCC------CGaCCGGGACUCUGCCCa -3' miRNA: 3'- -UGGGcauaccGC-GGCCUUGGGACGGG- -5' |
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31315 | 3' | -60.7 | NC_006623.1 | + | 145022 | 0.66 | 0.685319 |
Target: 5'- -aCCGUAcGG-GCCcGAACCCgaccGCCCu -3' miRNA: 3'- ugGGCAUaCCgCGGcCUUGGGa---CGGG- -5' |
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31315 | 3' | -60.7 | NC_006623.1 | + | 146379 | 0.71 | 0.411238 |
Target: 5'- aGCCCcauGUG-GGCGCCGGGGCgaUGCCg -3' miRNA: 3'- -UGGG---CAUaCCGCGGCCUUGggACGGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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