Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31317 | 3' | -53.8 | NC_006623.1 | + | 42121 | 0.66 | 0.927807 |
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Target: 5'- aCUGCuauuCAGAG-AGCCGCGUGCgGAUa- -3' miRNA: 3'- -GACG----GUCUUaUUGGCGCGCGaCUGaa -5' |
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| 31317 | 3' | -53.8 | NC_006623.1 | + | 137222 | 0.67 | 0.910269 |
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Target: 5'- -cGCCAGGAaguuCCGCGCGCguuuCUa -3' miRNA: 3'- gaCGGUCUUauu-GGCGCGCGacu-GAa -5' |
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| 31317 | 3' | -53.8 | NC_006623.1 | + | 114994 | 0.67 | 0.903916 |
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Target: 5'- -gGCCGGGAcuACCGCGUGCgcACUc -3' miRNA: 3'- gaCGGUCUUauUGGCGCGCGacUGAa -5' |
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| 31317 | 3' | -53.8 | NC_006623.1 | + | 146618 | 0.67 | 0.903916 |
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Target: 5'- -gGCCGGGAcuACCGCGUGCgcACUc -3' miRNA: 3'- gaCGGUCUUauUGGCGCGCGacUGAa -5' |
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| 31317 | 3' | -53.8 | NC_006623.1 | + | 117881 | 0.68 | 0.883366 |
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Target: 5'- -cGCCAGAGUGGCuCuaGCGC-GACa- -3' miRNA: 3'- gaCGGUCUUAUUG-GcgCGCGaCUGaa -5' |
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| 31317 | 3' | -53.8 | NC_006623.1 | + | 143730 | 0.68 | 0.883366 |
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Target: 5'- -cGCCAGAGUGGCuCuaGCGC-GACa- -3' miRNA: 3'- gaCGGUCUUAUUG-GcgCGCGaCUGaa -5' |
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| 31317 | 3' | -53.8 | NC_006623.1 | + | 106421 | 0.68 | 0.860673 |
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Target: 5'- -gGCCAGGcauAUGACUggaggaaugGCGUGUUGACUUa -3' miRNA: 3'- gaCGGUCU---UAUUGG---------CGCGCGACUGAA- -5' |
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| 31317 | 3' | -53.8 | NC_006623.1 | + | 124319 | 0.69 | 0.835995 |
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Target: 5'- gCUGCCGGAAUAguggccuggGCCGCG-GCgagGGCc- -3' miRNA: 3'- -GACGGUCUUAU---------UGGCGCgCGa--CUGaa -5' |
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| 31317 | 3' | -53.8 | NC_006623.1 | + | 137293 | 0.69 | 0.835995 |
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Target: 5'- gCUGCCGGAAUAguggccuggGCCGCG-GCgagGGCc- -3' miRNA: 3'- -GACGGUCUUAU---------UGGCGCgCGa--CUGaa -5' |
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| 31317 | 3' | -53.8 | NC_006623.1 | + | 27089 | 1.06 | 0.00586 |
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Target: 5'- uCUGCCAGAAUAACCGCGCGCUGACUUa -3' miRNA: 3'- -GACGGUCUUAUUGGCGCGCGACUGAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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